Development of New Evaluation Method for Biodegradation Behavior of Biodegradable Polymers by Multiple Analytical Measurements

通过多重分析测量开发可生物降解聚合物生物降解行为的新评价方法

• 批准号: 11650829
• 负责人: OHTANI Hajime
• 金额: $ 2.24万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650829/
• 关键词: Poly (ε-caprolactone); Biodegradation; End groups; Enzymatic degradation test; Polymer blend; Aliphatic polyesters; Pyrolysis-gas chromatography; Soil burialtest; 生分解性高分子; 分析的熱分解法; 酵素分解; 顕微赤外分光法; 水酸化テトラメチルアンモニウム

The enzymatic degradability of poly (lactic acid) [PLA]/ polycaprolactone [PCL] blend was evaluated focusing on the changes in the chemical compositions during its biodegradation. The almost equimolar blended samples were biodegraded in a phosphate buffer solution added with Pseudomonas sp. cholesterol esterase for 1-5 days. After this biodegradation procedure, the residual blend samples were subjected to Py-GC measurements in the presence of tetramethylammonium hydroxide (TMAH). Various methylesters and cyclic esters originating from each constituent polymer were sensitively observed on the pyrograms of the blend samples. The observed composition of the original blend estimated on the basis of their peak intensities was in good agreement with the feed composition. Moreover, the relative amounts of PCL constituent in the blend determined by reactive Py-GC slightly decreased with the time for the enzymatic degradation test, corresponding to the increase in the TOC in the buffer solution. This fact suggested that the biodegradation rate of PCL in the blend was faster than that of PLA.Furthermore, it is interesting to note that the degradation rate of PLA constituents in the blend, which was estimated from the weighed recovery and the observed composition by Py-GC,was much faster than that of pure PLA whereas the biodegradation of PCL constituent in the blend was significantly depressed as compared with that of pure PCL.This specific biodegradation behavior of the blended polyester can be correlated to its molphological "sea-island" structures.

研究了聚乳酸（PLA）/聚己内酯（PCL）共混物的酶降解性，重点考察了共混物在生物降解过程中化学组成的变化。将几乎等摩尔的混合样品在加入假单胞菌胆固醇酯酶的磷酸盐缓冲溶液中生物降解1-5天。在该生物降解程序之后，在四甲基氢氧化铵（TMAH）的存在下对残留共混物样品进行Py-GC测量。在共混物样品的热解图上灵敏地观察到源自每个组成聚合物的各种甲酯和环酯。观察到的组合物的原始混合物的基础上估计其峰强度是在良好的协议与饲料组成。此外，通过反应性Py-GC测定的共混物中PCL组分的相对量随着酶降解测试的时间略微降低，对应于缓冲溶液中TOC的增加。这一事实表明，共混物中PCL的生物降解速率比PLA快。此外，有趣的是，共混物中PLA成分的降解速率（根据称重回收率和Py-GC观察到的组成估计），共混物中PCL组分的生物降解速度比纯PLA快得多，而与纯PCL相比，共混物中PCL组分的生物降解明显受到抑制，共混聚酯的特定生物降解行为可与其形态学“海岛”结构相关。

项目成果

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H.Sato：“通过程序升温分析热解技术表征甲壳素基聚合物杂化物。2.甲壳素接枝聚(2-甲基-2-恶唑啉)/PVA”大分子。

H.Yokoi: "Discriminative analysis of Eicalyptus camaldulensis grown from seeds of various origins based on lignin components measured by pyrolysis-gas chromatography"Journal of Analytical and Applied Pyrolysis. 57. 145-152 (2001)

H.Yokoi：“基于热解-气相色谱法测量的木质素成分对不同来源种子生长的赤桉进行判别分析”分析与应用热解杂志。

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H.Sato: "A Novel Evaluation Method for Biodegradability of Poly(butylene succinate-co-butylene adipate) by Pyrolysis-Gas Chromatography"Polymer Degradation and Stability. (印刷中).

H. Sato：“通过热解-气相色谱法评估聚（琥珀酸丁二醇酯-己二酸丁二醇酯）生物降解性的新方法”聚合物降解和稳定性（印刷中）。

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