Development of a System Containing a Flow-Through Electrochemiluminescent Detector and Its Application to High-Sensitive Determination

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A system containing a flow-through electrochemiluminescent detector was developed for high-sensitive determination of biologically important substances. The main contents of the results are as follows :1. Construction of the electrochemiluminescent detection systemThe system developed was fabricated from a carrier solution reservoir, degasser, pump, injector of test solution, electrochemiluminescent cell and phototube. The experimental conditions, such as the composition of carrier solution, the size and length of Teflon tube for the flow line and the cell design, were optimized to give reproducible data with high sensitivity.2. High-sensitive determination of hydrogen peroxideDetermination of hydrogen peroxide was carried out using luminol, an electrochemiluminescent reagent. Hydrogen peroxide amount ranging from 59 fmol to 59 nmol could be determined using 5.0 μM luminol in a phosphate buffer (pH 7.6) at a flow rate of 0.4 ml/min and an applied potential of 1.1 V vs. Ag/AgCl.3. Analysis of serumAs examples of analysis of biological samples, glucose and uric acid in serum were determined. Glucose oxidase or uricase were immobilized on Chitopearl filled in an enzyme reactor tube located between the injector and detector. The present system should be applicable to a simple method for determining biologically important substances with high sensitivity.