Development of a System Containing a Flow-Through Electrochemiluminescent Detector and Its Application to High-Sensitive Determination

开发了一种具有流式电化学发光检测器的系统，用于高灵敏度测定生物重要物质。研究结果的主要内容如下：1。电化学发光检测系统由载体溶液储液池、脱气器、泵、测试溶液进样器、电化学发光电池和光电管组成。对载体溶液的组成、流动管道的聚四氟乙烯管的尺寸和长度以及电池的设计等实验条件进行了优化，以获得高灵敏度的重现性数据。过氧化氢的高灵敏度测定采用电化学发光试剂鲁米诺对过氧化氢进行了测定。在pH 7.6的磷酸盐缓冲液中，使用5.0 μM鲁米诺，流速0.4 ml/min，施加电位1.1 V vs. Ag/AgCl.3，可以测定59 ~ 59 nmol的过氧化氢量。血清分析作为生物样品分析的例子，测定了血清中的葡萄糖和尿酸。葡萄糖氧化酶或尿酸酶被固定在位于注射器和检测器之间的酶反应器管中填充的壳珠上。本系统应适用于测定具有高灵敏度的重要生物物质的简单方法。

A system containing a flow-through electrochemiluminescent detector was developed for high-sensitive determination of biologically important substances. The main contents of the results are as follows :1. Construction of the electrochemiluminescent detection systemThe system developed was fabricated from a carrier solution reservoir, degasser, pump, injector of test solution, electrochemiluminescent cell and phototube. The experimental conditions, such as the composition of carrier solution, the size and length of Teflon tube for the flow line and the cell design, were optimized to give reproducible data with high sensitivity.2. High-sensitive determination of hydrogen peroxideDetermination of hydrogen peroxide was carried out using luminol, an electrochemiluminescent reagent. Hydrogen peroxide amount ranging from 59 fmol to 59 nmol could be determined using 5.0 μM luminol in a phosphate buffer (pH 7.6) at a flow rate of 0.4 ml/min and an applied potential of 1.1 V vs. Ag/AgCl.3. Analysis of serumAs examples of analysis of biological samples, glucose and uric acid in serum were determined. Glucose oxidase or uricase were immobilized on Chitopearl filled in an enzyme reactor tube located between the injector and detector. The present system should be applicable to a simple method for determining biologically important substances with high sensitivity.