Development of a System for Determining a Small Amount of Acid Based on Electrolysis of Quinone

醌电解测定微量酸系统的研制

• 批准号: 10555295
• 负责人: KUSU Fumiyo
• 金额: $ 7.23万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10555295/
• 关键词: Determination of acid; Quinone; HPLC; Electrochemical detection; Human skin lipid; Sensor; Juice; Wine

An analytical system for determining a small amount of acid with high reliability and feasibility was developed. An acid detector for high-performance liquid chromatography (HPLC) and acid sensors were constructed and applied to various samples. The main contents of the results are as follows :1. High-sensitive detection system for free fatty acids (FFAs)A system with a high-sensitive detector was developed for FFA determination. In order to determine FFAs in human skin lipids and to clarify their distribution on skin surface, HPLC with this detector was carried out using an ODS separation column. This HPLC method was selective and high-sensitive and thus needed only a very simple sampling method. A piece of oil-absorbing paper (5 mm x 5 mm) was put on a sampling point of man's face. This procedure was useful for the research on FFA distribution on patient skin.2. Sensors for juice and wineAcid sensors were developed for the quality control of juice and wine, the taste of which strongly depends on acid content. The sensors were handy-type and functioned by common dry batteries. Several hundred microliters of juice or wine sample were added to a quinone solution in an electrochemical cell, and then the current value corresponded to acid content was measured within 60 s. The range of acid concentration determined by the sensors was from 0.1 to 3%. Acid contents of 20 samples were measured by the present and the conventional methods, and the correlation coefficient of those methods was 0.900. This sensor should thus prove quite useful for monitoring acid content of fruits and their products in fields or wineries.

开发了一种具有高可靠性和可行性的微量酸测定分析系统。构建了用于高效液相色谱 (HPLC) 的酸检测器和酸传感器，并将其应用于各种样品。主要研究结果如下： 1．游离脂肪酸 (FFA) 高灵敏度检测系统 开发了一种带有高灵敏度检测器的系统，用于 FFA 测定。为了测定人体皮肤脂质中的FFA并阐明其在皮肤表面的分布，使用该检测器并使用ODS分离柱进行了HPLC。该HPLC方法具有选择性和高灵敏度，因此只需要非常简单的采样方法。将一张吸油纸（5毫米×5毫米）放在人脸的采样点上。该方法对于研究FFA在患者皮肤上的分布具有一定的参考价值。 2．用于果汁和葡萄酒的传感器酸传感器是为了果汁和葡萄酒的质量控制而开发的，果汁和葡萄酒的味道很大程度上取决于酸含量。这些传感器是手持式的，由普通干电池供电。将数百微升果汁或酒样品添加到电化学电池中的醌溶液中，然后在60秒内测量与酸含量相对应的电流值。传感器测定的酸浓度范围为0.1%至3%。采用本方法和常规方法测定了20个样品的酸含量，相关系数为0.900。因此，该传感器对于监测田间或酿酒厂的水果及其产品的酸含量非常有用。

项目成果

A.Kotani,T.Fuse,and F.Kusu: "Determination of Plasma Free Fatty Acids by High-Performance Liquid Chromatography with Electrochemical Detection"Anal.Biochem.. 284. 65-69 (2000)

A.Kotani、T.Fuse 和 F.Kusu：“通过高效液相色谱法和电化学检测测定血浆游离脂肪酸”Anal.Biochem.. 284. 65-69 (2000)

S.Ohtsuki,N.Kunimatsu,K.Takamura,and F.Kusu: "Determination of the Total Acid Content in Wine Based on the Voltammetric Reduction of Quinone"Electroanalysis. (in press.).

S.Ohtsuki、N.Kunimatsu、K.Takamura 和 F.Kusu：“基于醌伏安还原法测定葡萄酒中的总酸含量”电分析。

S.Ohtsuki, N.Kunimatsu, K.Takamura, and F.Kusu: "Determination of the Total Acid Content in Wine Based on the Voltammetric Reduction of Quinone"Eletroanalysis. (in press). (2001)

S.Ohtsuki、N.Kunimatsu、K.Takamura 和 F.Kusu：“基于醌伏安还原法测定葡萄酒中的总酸含量”电分析。

K.Takamura,T.Fuse,K.Arai,F.Kusu: "A review of a new voltammetric method for determining acids"J.Electroanal.Chem.. 468. 53-63 (1999)

K.Takamura、T.Fuse、K.Arai、F.Kusu：“测定酸的新伏安法的综述”J.Electroanal.Chem.. 468. 53-63 (1999)

S.Ohtsuki,N.Kunimatsu,K.Takamura,and F.Kusu: "Determination of the Total Acid Content in Wine Based on the Voltammetric Reduction of Quinone"Electroanalysis. (in press).

S.Ohtsuki、N.Kunimatsu、K.Takamura 和 F.Kusu：“基于醌伏安还原法测定葡萄酒中的总酸含量”电分析。