Verification of Okuyama model for native collagen based on X-ray diffraction pattern obtained by synchrotron radiation

基于同步辐射获得的 X 射线衍射图验证天然胶原蛋白的 Okuyama 模型

• 批准号: 11650922
• 负责人: OKUYAMA Kenji
• 金额: $ 2.24万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11650922/
• 关键词: collagen; triple-helix; coi led-coil; fiber diffraction; x-ray diffraction; synchrotron radiation; crystal structure analysis; structural model; コラーゲン構造

The aim of this research project is to verify Okuyama model for collagen by using continuous diffraction obtained by synchrotron radiation. Unfortunately, we had only two chances to use synchrotron facility in the last year of this research term. Therefore, many of the things to do are not yet finished. The following results were obtained so far.(1) It was found that most of the specimens from kangaroo (or wallaby)-tail-tendon have a good orientation and good crystallinity k compared with those from rat-tail-tendon.(2) Since most of the diffraction spots from native collagen are very broad and diffuse, it is not possible to use background subtraction method usually used in the fiber diffraction field. We found that two-dimensional background subtraction by Fourier-Bessel function is the best method in the case of broad diffraction pattern from collagen.(3) Since we could use synchrotron facility only in the final year, we could not finish this research project within the research term. However, from the X-ray diffraction pattern taken by synchrotron radiation, we could say qualitatively that the Okuyama model for collagen could explain X-ray diffraction pattern from native collagen just as the Rich ＆ Crick model could. We will continue this research project to finish a quantitative analysis.

本研究的目的是利用同步辐射获得的连续衍射来验证胶原蛋白的Okuyama模型。不幸的是，在本研究学期的最后一年，我们只有两次机会使用同步加速器设备。因此，许多要做的事情还没有完成。结果表明：(1)与鼠尾腱相比，袋鼠(或袋鼠)尾腱的大部分样品具有良好的取向度和结晶度。(2)天然胶原的衍射点大多很宽且弥散，不能采用纤维衍射场中常用的背景减法。我们发现，在胶原蛋白衍射谱较宽的情况下，傅立叶-贝塞尔函数的二维背景减去是最好的方法。(3)由于我们只能在最后一年使用同步加速器设备，所以我们不能在研究期限内完成本研究项目。然而，从同步辐射X射线衍射图可以定性地说，胶原蛋白的Okuyama模型可以和Rich&Crick模型一样解释天然胶原蛋白的X射线衍射图。我们将继续这一研究项目，以完成量化分析。

项目成果

K. Okuyama: "Structural Study of Collagen Based on Single Crystal Analyses of Model Peptides In "Peptide Science 2000", ed. by T. Shioiri"The Japanese Peptide Society. 263-264 (2001)

K. Okuyama：“基于模型肽单晶分析的胶原蛋白结构研究”，《肽科学 2000》，T. Shioiri 编辑，日本肽学会。

K. Okuyama: "7/2-Helical model for collagen-Evidence from model peptides"Proc. Indian Acad. Sci.. 111. 19-34 (1999)

K. Okuyama：“胶原蛋白的 7/2 螺旋模型 - 来自模型肽的证据”Proc。

C. Hongo: "CrystaI structure analysis of a collagen-model peptide, (Pro-Pro-Gly) 9 at 1.0A resolution"Polymer J.. 33. 812-818 (2001)

C. Hongo：“1.0A 分辨率下胶原模型肽 (Pro-Pro-Gly) 9 的晶体结构分析”Polymer J.. 33. 812-818 (2001)

奥山健二: "コラーゲンの三重らせん構造"理学電気ジャーナル. 32. 12-23 (2001)

Kenji Okuyama：“胶原蛋白的三螺旋结构”Rigaku Electric Journal 32. 12-23 (2001)。