The Efferts of UV-A and UV-B radiation on the expression of genes in flower pigment biosynthesis

UV-A和UV-B辐射对花色素生物合成基因表达的影响

• 批准号: 11660029
• 负责人: KANECHI Michio
• 金额: $ 2.3万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660029/
• 关键词: Anthocyanin; Ultra violet radiation; Flower pigment; Transcription foctor; CHS; Flavonoid; UV

The color-changing rose 'Ehigasa' , which petals are redden after flowering under natural daylight, was investigate to clarify the effect of UV light quality (UV-A : 400-320 nm and UV-B : 320-280 nm) on flower pigment (anthocyanin) biosynthesis. Qualification of anthocyanidin skeleton and the bound sugars was investigated by separating of pigments and partial hydrolysates by PC and by measuring absorption spectrum. HPLC analysis of the total anthocyanin content and the ratio of main two anthocyanins to total anthocyanin content at differential developmental stages and light treatment. Main anthocyanins contained in petals of 'Ehigasa' were qualified as cyanidin 3,5-diglucosides (Cy-3G5G) and cyanidin 3-glucoside (Cy-3G). Biosynthesis of anthocyanin stimulated by UV was enhanced with increasing irradiation of visible light at the same time. Tendency of increasing ratio of Cy-3G was observed according to developing flower bud, and the quantity of Cy-3G was exceeded that of Cy-3G5G at unfolded.Leaf lettuce (cv. Red fire) which has colorful red pigment "anthocyanin" was treated with various UV supplemental irradiations, Chlorophyll and anthocyanin amount was much increased by UV irradiation with all wavelength. UV-B(>290 nm) radiation had no effect on the plant growth and the UV-B(>280 nm) affected leaf growth destructively. Even the low irradiance of UV-B280 was fatal. When the supplemental UV irradiation practiced at night only, the growth and development inhibited largely. While day and night irradiation result in no change in leaf color (the amount of pigments did not increased) but has no effect on growth.

以自然光下花瓣变红的变色玫瑰“Ehigasa”为材料，研究了不同光质（UV-A：400-320 nm和UV-B：320-280 nm）对花色素（花青素）合成的影响。通过对色素和部分水解产物的PC分离和吸收光谱的测定，对花青素骨架和结合糖进行了定性。不同发育阶段和光照处理下总花色素苷含量及主要两种花色素苷占总花色素苷的比例的HPLC分析。'Ehigasa'花瓣中的花色苷主要为花青素3，5-二糖苷（Cy-3G 5G）和花青素3-糖苷（Cy-3G）。紫外光促进花色素苷的生物合成，同时可见光照射量的增加也促进了花色素苷的生物合成。Cy-3G的含量随花蕾发育呈增加趋势，在展开期Cy-3G的含量超过Cy-3G 5G。用不同波长的紫外光辅助处理含有花色苷的红旗丹（红旗丹），结果表明，全波长的紫外光照射均能显著提高红旗丹的叶绿素含量和花色苷含量。UV-B（>290 nm）辐射对植株生长无影响，而UV-B（>280 nm）辐射对叶片生长有破坏性影响。即使UV-B 280的低辐照度也是致命的。当仅在夜间进行紫外线照射时，生长发育受到很大抑制。而昼夜照射对叶色无影响（色素含量不增加），但对生长无影响。