Molecular linkage map construction and mapping genes for useful traits in Asiatic hybrid lily

亚洲杂交百合有用性状分子连锁图谱构建及基因定位

• 批准号: 11660031
• 负责人: YAMAGISHI Masumi
• 金额: $ 1.92万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660031/
• 关键词: Asiatic hybrid lily; Anthocyanin; Carotenoids; Chalcone synthase (CHS); Dihydroflavonol 4-reductase (DFR); Lilium hybrids; Linkage map; QTL mapping; Asiatic hybrid lily; Myb; asiatic hybrid lily; Lilium sp; RAPDマーカー; ISSRマーカー; cDNAライブラリー; 花

To understand the genetic background of two floral anthocyanin pigmentation traits, anthocyanin pigmentation in the flower tepals and spot formation, in the Asiatic hybrid lily (2n=24), segregation of the two traits among 96 F_1 plants derived from a cross between commercial cultivars 'Montreux' and 'Connecticut King' were investigated. 'Montreux' has anthocyanin pigmentation in the tepals with many spots, and 'Connecticut King' has flowers with carotenoid pigmentation without spots. The F_1 plants with or without anthocyanin pigment in the tepals segregated with a 1 : 1 segregation ratio, indicating that a single gene controls anthocyanin pigmentation in the tepals. The number of spots per square centimeter of all tepals showed continuous distribution in the F_1 plants. To map the loci for the two anthocyanin pigmentation traits, molecular linkage maps in the Asiatic hybrid lily were constructed using a double pseudo-testcross strategy with the same F_1 plants used for phenotypic evaluation and 212 PCR-based DNA markers. The trait for anthocyanin pigmentation in tepals was used as a trait marker. The map of 'Montreux' comprised 95 markers in 26 linkage groups, and the map of 'Connecticut King' used 119 markers in 24 linkage groups. The total map lengths were 867.5 and 1114.8 cM, respectively. The trait locus for anthocyanin pigmentation in the tepals was between markers ASR35-180 and P506-40 in linkage group 1 of the 'Montreux' map with map distance 1.2 cM and 2.6 cM, respectively. A single point analysis of quantitative trait loci (QTL) for tepal spot number identified two putative QTLs in linkage groups 1 and 19 of the 'Connecticut King' map. One putative QTL in the linkage group 19 explained 64 % of the total phenotypic variation. Because both putative QTLs were mapped on the linkage map of 'Connecticut King' that has no spots, dominant alleles of them might suppress spot formation.

为了解亚洲百合(2n=24)花色苷着色性状的遗传背景，研究了亚洲百合(2n=24)以商品品种‘蒙特勒’和‘康涅狄格王’杂交产生的96株F1植株花色苷着色和斑点形成的遗传背景。‘蒙特勒’的花被片中有花青素的色素沉着，有许多斑点，而‘康涅狄格王’的花中有类胡萝卜素的色素沉着，没有斑点。花被片中有无花青素的F_1植株按1：1分离比例分离，表明花被片中花色素色素的形成是由单基因控制的。所有花被片每平方厘米的斑点数在F_1代植株中呈连续分布。为了定位这两个花色苷着色性状的基因座，采用双拟测交策略，利用212个DNA标记构建了亚洲百合F_1代的分子连锁图谱。花被片中花青素色素沉着的性状作为性状标记。‘蒙特勒’的图谱包括26个连锁群的95个标记，‘康涅狄格王’的图谱使用了24个连锁群的119个标记。图谱总长度分别为867.5和1114.8 cM。花被片花色苷着色的性状基因座位于‘蒙特勒’图谱连锁群1的标记ASR35-180和P506-40之间，图谱距离分别为1.2 cM和2.6 cM。通过对花被片斑点数的数量性状座位(QTL)的单点分析，在‘康涅狄格王’图谱的连锁群1和19上发现了两个可能的QTL。连锁群19中有1个可能的QTL解释了%的表型变异。由于这两个可能的QTL都定位在没有斑点的‘康涅狄格王’连锁图谱上，它们的显性等位基因可能会抑制斑点的形成。

项目成果

Hitomi Abe: "Genetic Analysis of Floral Anthocyanin Pigmentation Traits in Asiatic Hybrid Lily Using Molecular Linkage Maps"Theoretical and Applied Genetics. (in press). (2002)

阿部瞳：“利用分子连锁图谱对亚洲杂交百合花花青素色素沉着性状进行遗传分析”理论与应用遗传学。

Abe, Hiromi et al.: "Genetic analysis of floral anthocyanin pigmentation traits in Asiatic hybrid lily using molecular linkage maps"Theor. Appl. Genet.. (in press).

Abe、Hiromi 等人：“利用分子连锁图谱对亚洲杂交百合花花青素色素沉着性状进行遗传分析”理论。

Hiromi Abe: "Genetic Analysis of Floral Anthocyanin Pigmentation Traits in Asiatic Hybrid Lily using Molecular Linkage Maps"Theoretical and Applied Genetics. (in press). (2002)

Hiromi Abe：“利用分子连锁图谱对亚洲杂交百合花花青素色素沉着性状进行遗传分析”理论与应用遗传学。