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Characterization of MYB genes regulating anthocyanin biosynthesis in Asiatic hybrid lily

亚洲杂交百合 MYB 基因调控花色苷生物合成的特性

基本信息

批准号：
22580022
负责人：
YAMAGISHI Masumi
金额：
$ 2.41万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580022/
关键词：
スカシユリ花色遺伝子発現制御品種間差異形質発現

项目摘要

A single dominant locus determines anthocyanin biosynthesis in the tepals of Asiatic hybrid lilies(Lilium spp.); however the gene that determines this trait has not been previously reported. Furthermore, anthocyanin colour hue in tepals varies among cultivars, but the mechanisms underlying this variation are not known. Here, we show that LhMYB12, which is homologous to petunia An2, determines anthocyanin pigmentation in tepals and that the level of LhMYB12 transcription affects the quantity of anthocyanin pigments, producing colour hue variation in tepals. The pink-tepal cultivars 'Montreux' and 'Renoir' were heterozygous for the LhMYB12 gene. Their F_1 population segregated 3 : 1 for the presence or absence of anthocyanin in tepals, and LhMYB12 cosegregated perfectly with the presence of anthocyanin, indicating that LhMYB12 determines anthocyanin biosynthesis in tepals. Among 7 cultivars, a single anthocyanin cyanidin 3-0-0-rutinoside was detected in tepals displaying light pink, pink-red, dark red, or chocolate brown hues. However, concentrations differed according to the following order of colour hue : light pink<pink-red<dark red<chocolate brown, suggesting that colour hue variation in tepals is strongly related to anthocyanin quantities. The accumulation of LhMYB12 transcripts in tepals varied among 7 cultivars in the same order, and transcription levels were positively correlated with pigment quantities. This suggests that the levels of transcription of LhMYB12 vary among the different cultivars and subsequently affect the quantities of pigment in tepals. These results indicate that LhMYB12 determines anthocyanin pigmentation and generates the variation in anthocyanin-colour hue in lily tepals.

亚洲百合花被片花色素苷合成受一个显性基因控制;然而，决定这种性状的基因以前没有报道。此外，花被片中的花青苷色调因品种而异，但这种变化背后的机制尚不清楚。在这里，我们表明，LhMYB 12，这是同源的矮牵牛An 2，决定花色素苷色素在花被片和LhMYB 12的转录水平影响花色素苷色素的数量，产生色调变化的花被片。粉红色花被片品种“蒙特勒”和“雷诺阿”的LhMYB 12基因是杂合的。F_1代花被片中花色素苷的存在与否呈3：1分离，LhMYB 12与花色素苷的存在完全共分离，表明LhMYB 12决定着花被片中花色素苷的生物合成。在7个品种中，在花被片中检测到单一的花色素苷花青素3-0-0-芸香糖苷，显示浅粉红色，粉红色，暗红色或巧克力棕色色调。然而，浓度根据以下色调顺序而不同：浅粉红色<粉红色<暗红色<巧克力棕色，这表明花被片中的色调变化与花青素含量密切相关。7个品种中LhMYB 12转录本在花被片中的积累顺序相同，且转录水平与色素含量呈正相关。这表明LhMYB 12的转录水平在不同品种之间存在差异，并随后影响花被片中色素的数量。这些结果表明，LhMYB 12决定花色素苷色素沉着，并产生百合花被片花色素苷色调的变化。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

スカシユリ品種の'モントルー'と'ルノアール'の交雑に由来するF1集団におけるアントシアニン色素蓄積と肋MB12遺伝子の分離

决明百合品种“蒙特勒”和“雷诺阿”杂交 F1 群体中花色苷色素的积累和 rib MB12 基因的分离

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Yun-Song Lai;Yoshihiro Shimoyamada;Masayoshi Nakayama and Masumi Yamagishi;山崖璽登・赤木航一;Yun-Song Lai and Masumi Yamagishi;山岸真澄・吉田祐輔・中山真義
通讯作者：
山岸真澄・吉田祐輔・中山真義

Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development of Asiatic hybrid lily(Lilium spp.)

亚洲杂交百合花发育过程中色素积累及LhMYB12和花青素生物合成基因的转录