MECHANISM OF GENE INDUCTION IN ETHYLENE BIOSYNTHETIC PATHWAY BY HERBICIDES

除草剂乙烯生物合成途径基因诱导机制

• 批准号: 11660041
• 负责人: MATSUMOTO Hiroshi
• 金额: $ 2.11万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660041/
• 关键词: auxin-type herbicide; quinclorac; ethylene synthesis; bleaching; light; ACC synthase

Although the auxin-type are considered to herbicides act like natural auxins in plants and their stimulation of ethylene biosynthesis is assumed to be involved in the phytotoxic action of the herbicides, their precise action mechanism is still enigma. The objectives of this study were to clarify the mode of action of auxin-type herbicide quinclorac on susceptible gramineous plants and the mechanisms of gene induction involved in ethylene synthesis.Quinclorac treatment to corn (Zea mays cv. Honey Bantam) roots significantly stimulated ethylene evolution from shoots especially under light irradiation. Moreover, bleaching of leaves and decrease of water content was detected only in the light. Involvement of light for the phytotoxic action was unique to quinclorac and not observed with other auxin-type herbicides such as 2,4-D.Ethylene evolution was also affected by ambient temperature during the herbicide treatment.In this research, we found that ACC synthase, one of key enzymes of ethylene synthesis was significantly induced by quinclorac only in the light. Therefore induction of this enzyme is considered to be main factor of increased ethylene evolution. From the pattern of the induction, involvement of wound-inducible type ACC gene by the herbicide in the light was suggested. Investigation on the induced gene is now in progress.

虽然生长素类除草剂在植物中的作用类似于天然生长素，其对乙烯生物合成的刺激作用被认为与除草剂的植物毒性作用有关，但其确切的作用机制仍是个谜。本研究旨在阐明生长素型除草剂二氯喹啉酸对感病禾本科植物的作用方式和参与乙烯合成的基因诱导机制。根显著促进地上部的乙烯释放，特别是在光照下。此外，只有在光照下才能观察到叶片漂白和含水率下降的现象。光对二氯喹啉酸的植物毒性作用是独有的，而在其他生长素型除草剂如2，4-D中没有观察到。在除草剂处理过程中，环境温度也影响了乙烯的释放。在本研究中，我们发现二氯喹啉酸只在光下显著诱导乙烯合成的关键酶之一ACC合成酶的表达。因此，该酶的诱导被认为是增加乙烯释放的主要因素。从诱导模式来看，可能是光照下除草剂参与了创伤诱导型ACC基因的表达。对诱导基因的研究目前正在进行中。

项目成果

Sunohara,Y et al.: "Effect of temperature on quinclorac-induced ethylene production from corn leaves"J.Pesticide Sci.. 24・4. 375-380 (1999)

Sunohara, Y 等人：“温度对二氯喹啉酸诱导的玉米叶乙烯产生的影响”J.Pesticide Sci.. 24・4 (1999)。

小林真理,松本宏: "Quinclorac感受性イネ科植物におけるエチレン合成酵素活性の光誘導"雑草研究. 46(Suppl)(印刷中). (2001)

Mari Kobayashi、Hiroshi Matsumoto：“二氯乙烯合酶活性的光诱导”Weed Research 46（增刊）（印刷中）。

Sunohara, Y et al.: "Effect of temperature on quinclorac-induced ethylene production from corn leaves"J.Pesticide Sci.. 24. 375-380 (1999)

Sunohara, Y 等人：“温度对二氯喹啉酸诱导的玉米叶乙烯产生的影响”J.Pesticide Sci.. 24. 375-380 (1999)

Kobayashi, M.and Matsumoto, H.: "Light induction of ACC synthase activity in quinclorac treated susceptible species."Weed Research, Japan. 46 (Suppl.)(in press). (2001)

Kobayashi, M. 和 Matsumoto, H.：“二氯喹啉酸处理的易感物种中 ACC 合酶活性的光诱导。”杂草研究，日本。