Simulation of functional glycomolecule as carbohydrate model on cell surface

具有生物功能的碳水化合物存在于细胞表面，在细胞间相互作用和细胞识别中起重要作用。我们建立了一种简单的酶法合成以多聚谷氨酸为骨架、带有多价Lex的人工新糖肽和带有多价唾液酸寡糖的人工唾液酸糖肽的方法。前合成的糖肽被证明是有用的作为探针的碳水化合物识别和建模中的相互作用与凝集素的分析，通过使用表面等离子体共振分析仪BIAcore。寡糖作为侧链的多样性对诱导糖蛋白-凝集素相互作用的亲和力增强非常重要。后一种糖肽被阐明为对于研究A型和B型流感病毒株的唾液酸（SA）键特异性中和非常有用。这表明，新糖肽中的多价唾液酸糖单元显著提高了病毒中和活性。

Biologically functional carbohydrates exist on cell surface and play important roles on the intercellular interaction in cell-cell recognition. We developed a simple enzymatic method of the artificial neoglycopeptide with a poly(L-glutamic acid)s backbone carrying multivalent Lex as a mimic of the embryoglycan and the sialoglycopeptides carrying multivalent sialyl oligosaccharides as mimics of mucin-like glycopeptides. The former synthetic glycopeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of interaction with lectins by using a surface plasmon resonance analyzer BIAcore. The multiplicity of oligosaccharide as side chain was very important to induce the affity enhancement glycoprotein-lectin interaction. The latter glycopeptides were elucidated to be very useful for invesrigating the sialic acid (SA) linkage specific neutralization of influenza type A and B virus strains. It indicated that the multivalent sialyl sugar units in the neoglycopeptides drastically increase the viral neutralizing activities.