Simulation of functional glycomolecule as carbohydrate model on cell surface

细胞表面功能糖分子作为碳水化合物模型的模拟

• 批准号: 11660105
• 负责人: USUI Taichi
• 金额: $ 2.37万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660105/
• 关键词: Cell surface model; Carbohydrate interaction; Oligosaccharide unit; Molecular recognition; Simulation; Glycopolymer; Glycotechnology; Glycoconjugate; Carbohydrate interaction; Glycoll; Glycoprotein; Glycolipid; Glycotechnology; Carbohydrate p

Biologically functional carbohydrates exist on cell surface and play important roles on the intercellular interaction in cell-cell recognition. We developed a simple enzymatic method of the artificial neoglycopeptide with a poly(L-glutamic acid)s backbone carrying multivalent Lex as a mimic of the embryoglycan and the sialoglycopeptides carrying multivalent sialyl oligosaccharides as mimics of mucin-like glycopeptides. The former synthetic glycopeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of interaction with lectins by using a surface plasmon resonance analyzer BIAcore. The multiplicity of oligosaccharide as side chain was very important to induce the affity enhancement glycoprotein-lectin interaction. The latter glycopeptides were elucidated to be very useful for invesrigating the sialic acid (SA) linkage specific neutralization of influenza type A and B virus strains. It indicated that the multivalent sialyl sugar units in the neoglycopeptides drastically increase the viral neutralizing activities.

具有生物功能的碳水化合物存在于细胞表面，在细胞间相互作用和细胞识别中起重要作用。我们建立了一种简单的酶法合成以多聚谷氨酸为骨架、带有多价Lex的人工新糖肽和带有多价唾液酸寡糖的人工唾液酸糖肽的方法。前合成的糖肽被证明是有用的作为探针的碳水化合物识别和建模中的相互作用与凝集素的分析，通过使用表面等离子体共振分析仪BIAcore。寡糖作为侧链的多样性对诱导糖蛋白-凝集素相互作用的亲和力增强非常重要。后一种糖肽被阐明为对于研究A型和B型流感病毒株的唾液酸（SA）键特异性中和非常有用。这表明，新糖肽中的多价唾液酸糖单元显著提高了病毒中和活性。

项目成果

Kazuhide Totani: "Enzymatic synthesis of aliphatic β-lactosides as mimic units of glycosphingolipids by use of T.reesei cellulase"Arch. Biochem. Biophys.. 383. 28-37 (2001)

Kazuhide Totani：“利用里氏木霉纤维素酶酶促合成脂肪族 β-乳糖苷作为鞘糖脂的模拟单位”Biochem. 383. 28-37 (2001)

K. Totani, K. Shimizu, Y. Harada, T. Murata, T. Usui: "Enzymatic synthesis of oligosaccharide containing Lex unit by partially purified chicken serum"Biosci. Biotechnol. Biochem.. 66. 636-640 (2002)

K. Totani、K. Shimizu、Y. Harada、T. Murata、T. Usui：“通过部分纯化的鸡血清酶促合成含有 Lex 单元的寡糖”Biosci。

Tateomi Murata: "Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto-N-neofetraose"Glycoconjugate J.. 16. 189-195 (1999)

Tateomi Murata：“将乳糖轻松酶促转化为乳糖-N-四糖和乳糖-N-新四糖”Glycoconjugate J.. 16. 189-195 (1999)

X. Zeng, Y. Nakaaki, T. Murata, T. Usui: "Chemo-enzymatic synthesis of glycopeptides carrying α-Neu5Ac-(2-3)-β-D-Gal-(1-3)-α-D-GalNAc, β-D-Gal-(1-3)-α-D-GalNAc and related compounds and analysis of their specific interactions with lectins"Arch. Biochem. B

X. Zeng、Y. Nakaaki、T. Murata、T. Usui：“携带 α-Neu5Ac-(2-3)-β-D-Gal-(1-3)-α-D- 的糖肽的化学酶合成GalNAc、β-D-Gal-(1-3)-α-D-GalNAc 及相关化合物及其与凝集素的特异性相互作用分析"Arch. Biochem. B

Kazuhide Totani: "Enzymatic synthesis of oligosaccharide containing Lex unitby partially purified chicken serum"Biosci. Biotechnol. Biochem.. 66. 636-640 (2002)

Kazuhide Totani：“通过部分纯化的鸡血清酶促合成含有 Lex 单元的寡糖”Biosci。