Technological development of oligosaccharide units as biomaterials of life science

我们的目标是准备使用寡氯酮单元库，使生物学上重要的寡氯酮单元库的实用合成，以必要地用于开发糖基学和糖基化技术。β-D-Gal-D-GlcNAc和β-D-Gal-D-GalNAc中（1-3）、（1-4）和（1-6）链接的可能数组的合成是由某些可用的β-D-半乳糖苷制成的。将galactoyl-disaccharides单元库用于含有GlcNAc的酶合成; β-D-GlcNAC-（1-3）-β-D-Gal-（1-4）-GlcNAc作为聚乳酸-三氨基葡萄糖和β-D-Gal-（1 -3）-[β-D-GlcNAC-（1-6）]-α-D-GalNAc-OC-D26-D24-D2 NO-D22-p作为一个mucin型2核的碳氢化合物单元。Transglycosylation of L-Fuc to LacNAc and lactose utilizing？-L-fuosidases also produced？-L-fucosyl-N-acetylactosamine and 3'-O-\-L-fucosylactose. Furthermore，作为人类牛奶寡氯代化合物的综合战略，LACTO-N-四raose（β-D-Gal-（1-3）-β-D-GlcNac-（1-3）-β-D-Gal-（1-4）-D-Glc）和乳-N-neotraose（β-D-Gal-（1-4）-β-D-GlcNac-（1-3）-β-D-Gal-（1-4）-D-Glc）实际上是由糖基转移酶催化反应和糖基化酶的组合合成的。catalyzed transglycosylation from lactose as an initial substance.Such synthetic oligosaccharides available in large amounts were used to synthesize glycopolymer。糖基肽携带的LacNAc单元的化学-酶合成是作为乙酰葡萄糖蛋白模型的，而β-D-GalNAc和α-NeuAc-（1 - 3）-β-D-Gal-（1 - 3）-α-D-GalNAc单元则作为氨基酸型葡萄糖蛋白模型的，由引入这些黄色素作为侧链进入作为主链。人工糖基化蛋白质将被展示为有用的探针，即碳水化合物识别和建模在糖基化蛋白-lectin interactions的分析。

Our purpose is to prepare oligosaccharide units library folowing practical synthesis of biologically important oligosaccharides essentially for developing glycobology and glycottechnolog. The synthesis of possible array of linkages of (1-3), (1-4) and (1-6) of β-D-Gal-D-GlcNAc and β-D-Gal-D-GalNAc was done by using some readily available β-D-galactosidases. The preparation of the galactosyl-disaccharide units library led to the enzymatic synthesis of trisaccharides containing GlcNAc; β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-GlcNAc as a common unit of polylacto- saminoglycan and β-D-Gal-(1-3)-[β-D-GlcNAc-(1-6)]-α-D-GalNAc-OCィイD26ィエD2HィイD24ィエD2NOィイD22ィエD2-p as a carbohydrate unit of mucin type 2 core. Transglycosylation of L-Fuc to LacNAc and lactose utilizing α-L-fucosidases also produced α-L-fucosyl-N-acetyllactosamine and 3'-O-α-L-fucosyllactose. Furthermore, as a synthetic strategy of human milk oligosaccharide, lacto-N-tetraose (β-D-Gal-(1-3)-β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-D-Glc)and lacto-N-neotetraose(β-D-Gal-(1-4)-β-D-GlcNAc-(1-3)-β-D-Gal-(1-4)-D-Glc) were practically synthesized by a combination of glycosyltransferase catalyzed reaction and glycosidase-catalyzed transglycosylation from lactose as an initial substance.Such synthetic oligosaccharides available in large amounts were used to synthesize glycopolymer. chemo-enzymatic synthesis of glycopeptides carrying LacNAc unit as a model of asialoglycoprotein, and β-D-Gal-(1-3)-α-D-GalNAc and α-NeuAc-(1-3)-β-D-Gal-(1-3)-α-D-GalNAc units as models of mucin-type glycoproteins was carried out by introducing these oigosaccharides as side chain into polyglutamic acid as main chain. The artgificial glycopolypeptides were shown to be useful as probes of carbohydrate recognition and modeling in the analysis of glycoprotein-lectin interactions.