Preparation of novel material as adhesive biomatrix by devising oligosaccharide units

我们的目的是通过设计寡糖单元来创造一种作为粘合生物基质的新型材料。我们开发了糖多肽和糖脂作为人工糖结合物模拟物的合成方法，即通过化学酶法将寡糖单元引入多肽和甘油脂中。一、人工糖多肽（人工粘蛋白）的功能设计通过化学酶法合成了携带脱唾液酸型和唾液酸型粘蛋白聚糖的糖多肽，并被证明可用作工具和探针碳水化合物识别。它们还可用作人类流感病毒感染的聚合抑制剂。通过增加糖聚合物的分子量和唾液酸含量，显着增强病毒感染的能力。二、人工糖脂的功能设计设计并制备了由乳糖苷和磷脂组成的人工糖脂，作为乳糖神经酰胺的模拟物。乳糖基化的新糖脂很容易转化为糖脂质体，并且其乳糖残基有效地暴露在脂质体膜表面。我们的发现可能有助于改进定制糖脂质体的合理设计，作为研究生物识别现象的探针。

Our purpose is to create a novel material as adhesive biomatrix by devising oligosaccharide units. We developed the synthetic methods of glycopolypeptide and glycolipid as artificial glycosonjugate mimetics, which were chemo-enzymatically introduced oligosaccharide units to polypeptide and glycerolipid.I.Functional design of artificial glycopolypeptide (artificial mucin)Glycopolypeptides carrying the glycan of asialo-type and sialo-type mucins were chemo-enzymatically synthesized and shown to be useful as tools and probes of carbohydrate recognition. They were also useful as polymeric inhibitors of infection by human influenza viruses. Influenza of infection by viruses was remarkably enhanced by increasing the molecular weight and sialic acid content of glycopolymers.II.Functional design of artificial glycolipidArtificial glycolipids composed of lactose glycoside and phospholipids were designed and prepared as mimetics of lactosyl ceramide. The lactosylated neoglycolipids were easily transformed into glycoliposomes, and their lactose residues effectively exposed on the liposomal membrane surface. Our finding may contribute to an improved rational design of tailored glycoliposomes as probes for investigation of biological recognition phenomenon.