Preparation of novel material as adhesive biomatrix by devising oligosaccharide units

通过设计寡糖单元制备作为粘附生物基质的新型材料

• 批准号: 13556017
• 负责人: USUI Taichi
• 金额: $ 9.15万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13556017/
• 关键词: Glycotechnology; Glycoconjugate; Biomatrix; Enzymatic synthesis; Glycopeptide; Glycolipid; インフルエンザウイルス; リポソーム; Glycotechnology; Glycoconjugate; Moelcular design; Cell adhesion; Cellular matrix; Molecular recognition; Artificial glycomaterial

Our purpose is to create a novel material as adhesive biomatrix by devising oligosaccharide units. We developed the synthetic methods of glycopolypeptide and glycolipid as artificial glycosonjugate mimetics, which were chemo-enzymatically introduced oligosaccharide units to polypeptide and glycerolipid.I.Functional design of artificial glycopolypeptide (artificial mucin)Glycopolypeptides carrying the glycan of asialo-type and sialo-type mucins were chemo-enzymatically synthesized and shown to be useful as tools and probes of carbohydrate recognition. They were also useful as polymeric inhibitors of infection by human influenza viruses. Influenza of infection by viruses was remarkably enhanced by increasing the molecular weight and sialic acid content of glycopolymers.II.Functional design of artificial glycolipidArtificial glycolipids composed of lactose glycoside and phospholipids were designed and prepared as mimetics of lactosyl ceramide. The lactosylated neoglycolipids were easily transformed into glycoliposomes, and their lactose residues effectively exposed on the liposomal membrane surface. Our finding may contribute to an improved rational design of tailored glycoliposomes as probes for investigation of biological recognition phenomenon.

我们的目的是通过设计低聚糖单元来创造一种新型材料作为粘附生物基质。通过化学酶法将低聚糖单元引入到多肽和甘油脂中，开发了糖共肽和糖脂的合成方法。人工糖共肽（人工粘蛋白）的功能设计化学酶法合成了携带asialo型和sialo型粘蛋白聚糖的糖共肽，这些糖共肽可以作为碳水化合物识别的工具和探针。它们还可用作人类流感病毒感染的聚合抑制剂。通过增加糖共聚物的分子量和唾液酸含量，可以显著增强病毒感染的流行性感冒。2 .人工糖脂的功能设计设计并制备了由乳糖糖苷和磷脂组成的模拟乳糖神经酰胺的人工糖脂。乳糖化的新糖脂容易转化为糖脂质体，其乳糖残基有效地暴露在脂质体膜表面。我们的发现可能有助于改进糖脂质体的合理设计，以作为研究生物识别现象的探针。

项目成果

Kazuhide Totani: "Enzymatic synthesis of oligosaccharide comtaining Lex unit using partially purified chicken serum"Biosci.Biotech.Biochem.. 66. 636-640 (2002)

Kazuhide Totani：“使用部分纯化的鸡血清酶促合成含有 Lex 单元的寡糖”Biosci.Biotech.Biochem.. 66. 636-640 (2002)

Kazuhide Totani: "Enzymatic synthesis of oligosaccharide containing Lex unit by partially parified chicken serum"Biosci.Biotechnol.Biochem.. 66. 636-640 (2002)

Kazuhide Totani：“通过部分纯化的鸡血清酶促合成含有 Lex 单元的寡糖”Biosci.Biotechnol.Biochem.. 66. 636-640 (2002)

T.Murata, T.Hattori, S.Amarume, A.Koichi, T.Usui: "Kinetic studies on endo-p-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligo-Saccharide β-glycosides using its transglycosylation activity."Eur.J.Biochem.. 27

T.Murata、T.Hattori、S.Amarume、A.Koichi、T.Usui：“通过新型比色测定法对内切β-半乳糖苷酶进行动力学研究，并利用其合成 N-乙酰基乳糖胺重复寡糖 β-糖苷转糖基活性。”Eur.J.Biochem.. 27

Nozomu Yasutake: "Efficient synthesis of glyceroyl β-lactoside and its derivatives through a condensation reaction by cellulase"Biochim.Biophys.Acta. 1620. 252-258 (2003)

Nozomu Yasutake：“通过纤维素酶的缩合反应有效合成甘油酰基β-乳糖苷及其衍生物”Biochim.Biophys.Acta.1620.252-258(2003)

戸谷 一英: "人工ムチンの分子設計と機能解析"バイオサイエンスとバイオインダストリー. 60. 22-25 (2002)

Kazuhide Toya：“人工粘蛋白的分子设计和功能分析”生物科学和生物工业。 60. 22-25 (2002)。