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Study of fish cultivation using carotenoids supplemented diets without medication

类胡萝卜素添加饲料不用药养鱼的研究

基本信息

批准号：
11660192
负责人：
TACHIBANA Katsuyasu
金额：
$ 1.98万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Red sea bream and tiger puffer larvae were fed with carotenoids(β-carotene or astaxanthin) supplemented diets or unsupplemented control diet for 20 and 28 days after hatchout, respectively without medication. Results show that survival rates of carotenoids supplemented groups of both red sea bream and tiger puffer were higher than that of each control group. The carotenoids supplemented and unsupplemented control groups exhibited a similar growth in both species during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from β-carotene supplemented or astaxanthin supplemented group were higher than that of a control group in red sea bream. In tiger puffer, splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg /ml of Con A from the β-carotene-supplemented group. The lymphocytes proliferated to a higher degree compared to the control. Yellowtail (1-year-old) were fed with β-carotene-supplemented diets or unsupplemented control diet for 2-3 months without medication. Results show that the p-carotene-supplemented and unsupplemented control groups exhibited a similar growth and a similar serum values in Ht, Hb, GOT, GPT during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from p-carotene supplemented group was higher than that of a control group.Results suggested that the supplementation of carotenoids (β-carotene or astaxanthin) to diets, might be of benefit in production of healthy and high resistive fishes against infectious disease without medication.

红鲷鱼和虎河鲀幼虫在孵化后分别用类胡萝卜素（β-胡萝卜素或虾青素）补充饲料或未补充的对照饲料喂养20天和28天，不用药。结果表明，添加类胡萝卜素的红鲷组和虎河豚组的存活率均高于各对照组。在本实验中，补充类胡萝卜素和未补充类胡萝卜素的对照组在两个物种中表现出相似的生长。补充β-胡萝卜素或虾青素组的20μg/ml商陆丝裂原或100μg/ml刀豆球蛋白A(Con A)处理的红鲷的脾细胞增殖均高于对照组。在虎鲀中，脾细胞用 20 μg/ml 商陆有丝分裂原或 100 μg/ml 来自补充 β-胡萝卜素组的 Con A 处理。与对照相比，淋巴细胞增殖程度更高。鰤鱼（1岁）在不用药的情况下饲喂添加β-胡萝卜素的日粮或未添加的对照日粮2-3个月。结果表明，在本实验中，补充β-胡萝卜素的对照组和未补充β-胡萝卜素的对照组表现出相似的生长以及相似的血清Ht、Hb、GOT、GPT值。 20 μg/ml 商陆有丝分裂原或 100 μg/ml 刀豆球蛋白 A (Con A) 处理的 p-胡萝卜素添加组的脾细胞增殖率高于对照组。结果表明，在日粮中添加类胡萝卜素（β-胡萝卜素或虾青素）可能有利于生产健康且高抗性的鱼类。无需药物治疗的传染病。