Study of fish cultivation using carotenoids supplemented diets without medication

在不给药的情况下，分别饲喂类胡萝卜素（β-胡萝卜素或虾青素）添加饲料和不添加对照饲料，饲养20 d和28 d。结果表明，类胡萝卜素补充组和红鲷鱼组的成活率均高于对照组。在这个实验中，类胡萝卜素补充组和未补充组在两个物种中表现出相似的生长。20 μg/ml刺草丝裂原或100 μg/ml添加β-胡萝卜素或虾青素的刀豆蛋白A （Con A）对红鲷脾细胞的增殖作用均高于对照组。用20 μg/ml刺草丝裂原或100 μg/ml β-胡萝卜素补充组的Con A处理虎鲀脾细胞。与对照组相比，淋巴细胞增殖程度更高。1岁黄尾鱼分别饲喂β-胡萝卜素添加饲料或对照组饲料，饲喂2-3个月，不给药。结果表明，在实验过程中，p-胡萝卜素添加组和未添加组的生长情况相似，血清中Ht、Hb、GOT、GPT值相似。20 μg/ml刺草丝裂原或100 μg/ml p-胡萝卜素添加组脾细胞增殖均高于对照组。结果表明，在饲料中添加类胡萝卜素（β-胡萝卜素或虾青素）可能有利于生产健康且对传染病具有高抵抗力的鱼类，而无需药物治疗。

Red sea bream and tiger puffer larvae were fed with carotenoids(β-carotene or astaxanthin) supplemented diets or unsupplemented control diet for 20 and 28 days after hatchout, respectively without medication. Results show that survival rates of carotenoids supplemented groups of both red sea bream and tiger puffer were higher than that of each control group. The carotenoids supplemented and unsupplemented control groups exhibited a similar growth in both species during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from β-carotene supplemented or astaxanthin supplemented group were higher than that of a control group in red sea bream. In tiger puffer, splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg /ml of Con A from the β-carotene-supplemented group. The lymphocytes proliferated to a higher degree compared to the control. Yellowtail (1-year-old) were fed with β-carotene-supplemented diets or unsupplemented control diet for 2-3 months without medication. Results show that the p-carotene-supplemented and unsupplemented control groups exhibited a similar growth and a similar serum values in Ht, Hb, GOT, GPT during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from p-carotene supplemented group was higher than that of a control group.Results suggested that the supplementation of carotenoids (β-carotene or astaxanthin) to diets, might be of benefit in production of healthy and high resistive fishes against infectious disease without medication.