Effects of feeding of beta-carotene supplemented rotifers on anti viral activity of fish larva

用添加β-胡萝卜素的轮虫和未添加β-胡萝卜素的对照轮虫饲养真鲷（Pagrus major）、日本鹦嘴鱼（Oplegnathus fasciatus）和斑点鹦嘴鱼（Oplegnathus punctatus）仔鱼2 4-30 d。结果表明，添加n-胡萝卜素的真鲷、日本鹦嘴鱼和斑点鹦嘴鱼仔鱼的成活率均高于对照组。在本实验中，添加β-胡萝卜素和未添加β-胡萝卜素的对照组在所有物种中均表现出相似的生长。添加β-胡萝卜素组的100 μ g ml/L ConA、10或50 μ g ml/L商陆有丝分裂原的脾淋巴细胞增殖高于对照组日本鹦嘴鱼。<-1><-1>在用100 μ g/ml来自β-胡萝卜素补充组的ConA处理的红鲷和斑点鹦嘴鱼中，与对照组相比，淋巴细胞增殖程度更高。<-1>结果表明，在轮虫中添加β-胡萝卜素，可能有利于培育出健康、抗病能力强的轮虫幼虫。

Red sea bream (Pagrus major) Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish (Oplegnathus punctatus) larvae were fed with beta-carotene supplemented rotifers or unsupplemented control rotifers for 2 4-30 days after hatch out. Results showed that survival rates of n-carotene supplemented groups of Red sea bream, Japanese parrotfish, and Spotted parrotfish larvae were higher than that of control groups. p-carotene supplemented and unsupplemented control groups exhibited similar growth in all species during this experiment.Proliferation of spleen lymphocyte with 100mug ml^<-1> of ConA, 10 or 50mug ml^<-1> of Poke weed mitogen from beta-carotene supplemented group was higher than that of control group in Japanese parrotfish. In Red sea bream and Spotted parrotfish treated with 100mug ml^<-1> of ConA from the beta-carotene supplemented groups lymphocytes proliferated to a higher degree compared with control. Results suggested that the supplementation of beta-carotene to rotifers, might be of benefit in production of healthy and high resistive larvae against infectious disease.