Effects of feeding of beta-carotene supplemented rotifers on anti viral activity of fish larva

饲喂添加β-胡萝卜素的轮虫对鱼类幼虫抗病毒活性的影响

• 批准号: 08660234
• 负责人: TACHIBANA Katsuyasu
• 金额: $ 0.58万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660234/
• 关键词: beta-carotene; Red sea bream; Japanese parrotfish; Spotted parrotfish; lymphocytes; rotifer; larvae; virus infection; 幼若化; 脾臓リンパ球; 稚魚

Red sea bream (Pagrus major) Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish (Oplegnathus punctatus) larvae were fed with beta-carotene supplemented rotifers or unsupplemented control rotifers for 2 4-30 days after hatch out. Results showed that survival rates of n-carotene supplemented groups of Red sea bream, Japanese parrotfish, and Spotted parrotfish larvae were higher than that of control groups. p-carotene supplemented and unsupplemented control groups exhibited similar growth in all species during this experiment.Proliferation of spleen lymphocyte with 100mug ml^<-1> of ConA, 10 or 50mug ml^<-1> of Poke weed mitogen from beta-carotene supplemented group was higher than that of control group in Japanese parrotfish. In Red sea bream and Spotted parrotfish treated with 100mug ml^<-1> of ConA from the beta-carotene supplemented groups lymphocytes proliferated to a higher degree compared with control. Results suggested that the supplementation of beta-carotene to rotifers, might be of benefit in production of healthy and high resistive larvae against infectious disease.

用添加β-胡萝卜素的轮虫和未添加β-胡萝卜素的对照轮虫饲养真鲷（Pagrus major）、日本鹦嘴鱼（Oplegnathus fasciatus）和斑点鹦嘴鱼（Oplegnathus punctatus）仔鱼2 4-30 d。结果表明，添加n-胡萝卜素的真鲷、日本鹦嘴鱼和斑点鹦嘴鱼仔鱼的成活率均高于对照组。添加β-胡萝卜素的对照组和添加β-胡萝卜素的对照组在本实验中表现出相似的生长，添加β-胡萝卜素的对照组在100 μ g/<-1>ml ConA、10或50 μ g/<-1>ml商陆有丝分裂原的情况下，日本鹦嘴鱼脾淋巴细胞的增殖率高于对照组。在用100 μ g/ml来自<-1>β-胡萝卜素补充组的ConA处理的红鲷和斑点鹦嘴鱼中，与对照组相比，淋巴细胞增殖程度更高。结果表明，在轮虫中添加β-胡萝卜素，可能有利于培育出健康、抗病能力强的轮虫幼虫。

项目成果

K.Tachibana, M.Yagi, K.Hara, T.Mishima, & M.Tsuchimot: "Effects of feeding of beta-carotene supplemented rotifers on survival and lymphocytes proliferation reaction of fish larvae (Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish (Oplegn

K.Tachibana、M.Yagi、K.Hara、T.Mishima、