Effects of feeding of beta-carotene supplemented rotifers on anti viral activity of fish larva

用添加β-胡萝卜素的轮虫和未添加的对照轮虫孵化2、4~30d后，对团头鱼、斑点罗非鱼和斑点罗非鱼幼体进行摄食。结果表明，添加正胡萝卜素的团头鱼、日本鹦鹉鱼和斑点鹦鹉仔鱼的存活率均高于对照组。添加β-胡萝卜素和不添加β-胡萝卜素的对照组在所有物种中的生长情况相似。添加β-胡萝卜素的ConA组的脾淋巴细胞增殖能力高于对照组，而添加β-胡萝卜素组的日本鹦鹉的脾淋巴细胞增殖能力高于对照组。添加β-胡萝卜素100 mg·L~(-1)~(-1)·g~(-1)的β-胡萝卜素处理组的红鱼和斑点鹦鹉的淋巴细胞增殖程度高于对照组。结果表明，在轮虫中添加β-胡萝卜素，可能有利于生产健康的、对传染病具有高抗性的幼虫。

Red sea bream (Pagrus major) Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish (Oplegnathus punctatus) larvae were fed with beta-carotene supplemented rotifers or unsupplemented control rotifers for 2 4-30 days after hatch out. Results showed that survival rates of n-carotene supplemented groups of Red sea bream, Japanese parrotfish, and Spotted parrotfish larvae were higher than that of control groups. p-carotene supplemented and unsupplemented control groups exhibited similar growth in all species during this experiment.Proliferation of spleen lymphocyte with 100mug ml^<-1> of ConA, 10 or 50mug ml^<-1> of Poke weed mitogen from beta-carotene supplemented group was higher than that of control group in Japanese parrotfish. In Red sea bream and Spotted parrotfish treated with 100mug ml^<-1> of ConA from the beta-carotene supplemented groups lymphocytes proliferated to a higher degree compared with control. Results suggested that the supplementation of beta-carotene to rotifers, might be of benefit in production of healthy and high resistive larvae against infectious disease.