Effects of feeding of beta-carotene supplemented rotifers on anti viral activity of fish larva

赤鲷（Pagrus major）、日本鹦嘴鱼（Oplegnathus fasciatus）和斑点鹦嘴鱼（Oplegnathus punctatus）幼虫在孵化后2 ~ 30 d分别饲喂添加β -胡萝卜素的轮虫和未添加β -胡萝卜素的对照轮虫。结果表明，n-胡萝卜素添加组红鲷鱼、日本鹦嘴鱼和斑点鹦嘴鱼的仔鱼存活率均高于对照组。p-胡萝卜素添加组和未添加组在试验期间所有物种的生长情况相似。β -胡萝卜素添加组ConA浓度为100 μ g ml^<-1>， Poke weed有丝分裂原浓度为10、50 μ g ml^<-1>时，日本鹦嘴鱼脾脏淋巴细胞增殖均高于对照组。在红海鲷和斑点鹦嘴鱼中，添加了100马克毫升^<-1百万分之一的β -胡萝卜素组的ConA后，淋巴细胞的增殖程度高于对照组。结果表明，在轮虫体内补充β -胡萝卜素可能有利于生产健康的、对传染病具有高抵抗力的幼虫。

Red sea bream (Pagrus major) Japanese parrotfish (Oplegnathus fasciatus) and Spotted parrotfish (Oplegnathus punctatus) larvae were fed with beta-carotene supplemented rotifers or unsupplemented control rotifers for 2 4-30 days after hatch out. Results showed that survival rates of n-carotene supplemented groups of Red sea bream, Japanese parrotfish, and Spotted parrotfish larvae were higher than that of control groups. p-carotene supplemented and unsupplemented control groups exhibited similar growth in all species during this experiment.Proliferation of spleen lymphocyte with 100mug ml^<-1> of ConA, 10 or 50mug ml^<-1> of Poke weed mitogen from beta-carotene supplemented group was higher than that of control group in Japanese parrotfish. In Red sea bream and Spotted parrotfish treated with 100mug ml^<-1> of ConA from the beta-carotene supplemented groups lymphocytes proliferated to a higher degree compared with control. Results suggested that the supplementation of beta-carotene to rotifers, might be of benefit in production of healthy and high resistive larvae against infectious disease.