Local regulatory mechanisms of ovarian function by a cross-talk between vascular endothelial cells and ovarian cells : a mechanism of ovulation and corpus luteum formation.

通过血管内皮细胞和卵巢细胞之间的串扰来调节卵巢功能的局部调节机制：排卵和黄体形成的机制。

• 批准号: 11660276
• 负责人: MIYAMOTO Akio
• 金额: $ 2.3万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660276/
• 关键词: Ovulation; CL formation; Prostaglandin; Angiotensin II; Atrial natriuretic peptide; Endothelial cells; Steroidogenic cells; Cow; アンギオテンシン 2; 血管新生; プロジェステロン; 生体微透析システム

In mammalian females, the ovary plays a central role as an endocrine gland as well as the site of germ cell production. This study focused on the paracrine/autocrine mechanisms for local regulation of ovulation and subsequent formation and regression of the corpus luteum (CL) in the cow and ewe For this purpose, a microdialysis system (MDS) was developed for follicle and the CL both in vivo and in vitro. This technique enables us to observe real-time changes in levels of intercellular transmitters in the ovary during a "death" and a "new formation" of follicle and the CL.In addition, the exposure of ovarian cells with bioactive molecules through MDS resulted in different responses seen in cell cultures, suggesting the importance of intact cell-to-cell contact. After the LH-surge, intrafollicular prostaglandin (PG) and angiotensin II (Ang II) production and secretion acutely increased, and ovulation occurred. During the early development of new CL after the ovulation, local PG and Ang II secretions are maintained at high levels, indicating a direct relationship between active secretion of these factors and a rapid angiogenesis in the CL.The results from in vitro studies led us to propose the concept that a local regulatory mechanism of angiogenesis also enhances the capacity of luteal cells to synthesize/secrete progesterone (P). The overall results of the study spotlight the impact of the cross-talk between vascular endothelial cells and ovarian steroidogenic cells via vasoactive peptides and PG at ovulation and CL formation/regression in the ovary.

在哺乳动物雌性中，卵巢作为内分泌腺和生殖细胞产生的场所起着中心作用。本研究主要研究奶牛和母羊体内卵泡和黄体（CL）形成和消退的旁分泌/自分泌局部调控机制。为此，研制了体内和体外的卵泡和黄体微透析系统（MDS）。这项技术使我们能够实时观察卵巢中细胞间递质水平在卵泡和卵细胞“死亡”和“新形成”期间的变化。此外，通过MDS暴露具有生物活性分子的卵巢细胞会在细胞培养中产生不同的反应，这表明细胞间完整接触的重要性。lh激增后，前列腺素（PG）和血管紧张素II （Ang II）的产生和分泌急剧增加，并发生排卵。在排卵后新CL的早期发育过程中，局部PG和Ang II的分泌维持在较高水平，表明这些因子的活跃分泌与CL的快速血管生成有直接关系。体外研究的结果使我们提出了血管生成的局部调节机制也增强了黄体细胞合成/分泌黄体酮(P)的能力的概念。本研究的总体结果强调了血管内皮细胞和卵巢类固醇生成细胞之间通过血管活性肽和PG在卵巢排卵和CL形成/消退中的串扰的影响。

项目成果

Hayashi K,Miyamoto A,Berisha B,Kosmann MR,Okuda K and Schams D.: "Regulation of angiotensin II production and angiotensin receptors in microvascular endothelial cells from bovine corpus luteum."Biology of Reproduction. 62. 162-167 (2000)

Hayashi K、Miyamoto A、Berisha B、Kosmann MR、Okuda K 和 Schams D.：“牛黄体微血管内皮细胞中血管紧张素 II 产生和血管紧张素受体的调节。”生殖生物学。

Levy N,Kobayashi S,Roth Z,Wolfenson D,Miyamoto A and Meidan R.: "Administration of prostaglandin F2α during the early bovine luteal phase does not alter the expression of ET-1 and of its type a receptor : A possible cause for corpus luteum refractoriness.

Levy N、Kobayashi S、Roth Z、Wolfenson D、Miyamoto A 和 Meidan R.：“在牛黄体期早期施用前列腺素 F2α 不会改变 ET-1 及其 a 型受体的表达：可能的原因黄体不应性。

Hayashi K.et al.: "Absence of angiotensin II production and its action on secretory function in bovine luteinized granulosa cells"Journal of Reproduction and Development. 45(5). 357-361 (1999)

Hayashi K.等人：“牛黄素化颗粒细胞中血管紧张素 II 生成的缺失及其对分泌功能的作用”生殖与发育杂志。

Levy N,Kobayashi S,Roth Z,Wolfenson D,Miyamoto A and Meidan R.: "Administration of prostaglandin F2α during the early bovine luteal phase does not alter the expression of ET-1 and its type A receptor : A possible cause for corpus luteum refractoriness"Bio

Levy N、Kobayashi S、Roth Z、Wolfenson D、Miyamoto A 和 Meidan R.：“在牛黄体期早期施用前列腺素 F2α 不会改变 ET-1 及其 A 型受体的表达：一个可能的原因黄体耐火度"Bio

Levy N, Kobayashi S, Roth Z, Wolfenson D, Miyamoto A and Meidan R.: "Administration of prostaglandin F2α during the early bovine luteal phase does not alter the expression of ET-1 and its type A receptor : A possible cause for corpus luteum refractoriness

Levy N、Kobayashi S、Roth Z、Wolfenson D、Miyamoto A 和 Meidan R.：“在牛黄体期早期施用前列腺素 F2α 不会改变 ET-1 及其 A 型受体的表达：一个可能的原因黄体不应期