Development of Technology for Regulating Reproductive Efficiency by Controlling Corpus Luteum Function in Cows

控制奶牛黄体功能调节繁殖效率技术开发

• 批准号: 12556046
• 负责人: MIYAMOTO Akio
• 金额: $ 5.31万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12556046/
• 关键词: Corpus luteum; Angiogenesis; Prostaglandin; Vasoactive peptide; Blood flow; Progesterone; hCG; Cow; 血管構築因子; 成長因子; アンギオテンシン 2; 成長ホルモン; オキシトシン

The ovarian function is controlled by vascular functions in various local phenomena including ovulation, corpus luteum (CL) development and regression. This study was conducted to develop a basic as well as applicable technology to activate CL angiogenesis and function thereby improving the conception rate by keeping high progesterone level in the cow. At the same time, a local secretion by a microdialysis system together with a local blood flow by a color Doppler ultrasonography observation of ovulatory follicle and CL during development and regression was examined in detail in vivo. From the series of experiments, the following points were clarified for the first time.1. A LH surge activates a complex of local secretion of PG and vasoactive peptides that induces an acute increase in the local blood flow within the preovulatory follicular wall, but not within the atretic follicles. These changes are essential process to ovulation. Consequently, we proposed the existence of endothelin- … More angiotensin-ANP system in the preovulatory follicles.2. The active angiogenesis occurring immediately after ovulation directly relates to the formation of CL and its function (progesterone secretion), and this local mechanism involves secretory function of PG, angiotensin and ANP, but not for endothelin. Particularly, a luteolytic factor PGF2α produced in the developing CL play a role as a luteotropic rather than luteolytic factor. This unique mechanism may directly correlate with anti-luteolytic characteristic of developing CL.3. Once the CL matures, PGF2α can induce luteolysis in which other vasoactive peptides such as endothelin and angiotensin are highly active and luteolytic. This local mechanism induces a serious inhibition of local blood flow within the CL so that a cascade of luteolysis is accelerated further.4. A single injection of hCG to the cow one day after ovulation effectively activates the CL formation as well as plasma progesterone level, possible by promoting angiogenesis in the CL. To confirm the impact of this treatment on the conception rate after artificial insemination and embryo transfer, the field studies are also on going. Less

卵巢功能受血管功能的控制，在各种局部现象中包括排卵、黄体（CL）的发育和消退。本研究旨在开发一种基础的、可应用的技术来激活CL血管生成和功能，从而通过保持奶牛体内高孕酮水平来提高受胎率。同时，通过微透析系统的局部分泌以及通过彩色多普勒超声观察排卵卵泡和CL在发育和消退过程中的局部血流进行了详细的体内检查。通过一系列实验，首次阐明了以下几点. LH峰激活PG和血管活性肽的局部分泌复合物，其诱导排卵前卵泡壁内局部血流量的急性增加，但不包括闭锁卵泡内。这些变化是排卵的基本过程。因此，我们提出内皮素的存在- ...更多信息 排卵前卵泡的血管紧张素-心钠素系统.排卵后立即发生的活跃的血管生成与CL的形成及其功能（孕酮分泌）直接相关，这种局部机制涉及PG、血管紧张素和ANP的分泌功能，而不涉及内皮素的分泌功能。特别是，发育中的CL中产生的溶黄体因子PGF 2 α发挥促黄体因子而不是溶黄体因子的作用。这种独特的机制可能与发展中的CL的抗黄体溶解特性直接相关。一旦CL成熟，PGF 2 α可诱导黄体溶解，其中其他血管活性肽如内皮素和血管紧张素具有高度活性和黄体溶解性。这种局部机制导致CL内局部血流的严重抑制，从而进一步加速黄体溶解的级联反应。在排卵后一天向奶牛单次注射hCG有效地激活CL形成以及血浆孕酮水平，这可能是通过促进CL中的血管生成。为了证实这种治疗对人工授精和胚胎移植后受孕率的影响，实地研究也在进行中。少

项目成果

Kobayashi S, Acosta TJ, Ozawa T, Hayashi K, Berisha B, Ohtani M, Schams D, Miyamoto A: "Intraluteal release of angiotensin II and progesterone in vivo during corpora lutea development in the cow : effect of vasoactive peptides"Biology of Reproduction. 66.

Kobayashi S、Acosta TJ、Ozawa T、Hayashi K、Berisha B、Ohtani M、Schams D、Miyamoto A：“牛黄体发育期间体内血管紧张素 II 和黄体酮的黄体内释放：血管活性肽的作用”生殖生物学

Hayashi K, Acosta TJ, Bersha B, Kobayashi S, Ohtani M, Schams D, Miyamoto A: "Changes in prostaglandin secretion by the regressing bovine corpus luteum"Prostaglandins & Other Lipid Mediators. 70. 339-349 (2003)

Hayashi K、Acosta TJ、Bersha B、Kobayashi S、Ohtani M、Schams D、Miyamoto A：“牛黄体退化引起的前列腺素分泌变化”前列腺素

Kobayashi S: "Intraluteal release of prostaglandin F_<2α> and E_2 during corpora lutea development in the cow"Journal of Reproduction and Development. 48. 583-590

Kobayashi S：“牛黄体发育过程中前列腺素 F_<2α> 和 E_2 的黄体内释放”《生殖与发育杂志》48. 583-590。

Berisha B: "The expression of angiotensin and endothelin system members in bovine corpus luteum during estrous cycle and pregnancy"Endocrine. 19. 305-312

Berisha B：“动情周期和妊娠期间牛黄体中血管紧张素和内皮素系统成员的表达”内分泌。

Hayashi K: "Changes in prostaglandin secretion by the regressing bovine corpus luteum"Prostaglandins & Other Lipid Mediators. 70. 339-349

Hayashi K：“牛黄体退化引起的前列腺素分泌变化”前列腺素