A role of NO on neuroendocrine system : molecular, pharmacological and neurophysiological approaches
NO 对神经内分泌系统的作用:分子、药理学和神经生理学方法
基本信息
- 批准号:11670078
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
NO is thought to have important roles as a factor of cross-talk with cell-to-cell communication in not only peripheral system but also central nervous system. The purpose of the present study was to examine the physiological role on the neuroendocrine system that integrates autonomic nervous function and endocrine. Patch-clamp recording from the rat supraoptic nucleus (SON) in vitro demonstrated that inhibitory postsynaptic currents were increased by application of NO donor. For nociceptive stimulation, formalin (5%) or saline was injected subcutaneously (s.c.) into the bilateral hind paws of rats. The expression of the neuronal NO synthase (nNOS) gene in the paraventricular nucleus (PVN) was significantly increased 2 and 6 h after s.c. injection of formalin in comparison with that in untreated and saline injected rats. Food deprivation for 2 days caused downregulation of the expression of the nNOS gene in the PVN and the SON of rats. Central administration of leptin prevented food deprivation-induced that downregulation. In lithium-induced diabetes insipidus rats nNOS mRNA and NADPH-diaphorase activity were increased in the PVN and the SON.The levels of nNOS mRNA and NADPH-diaphorase activity in the PVN and SON of hypertensive Dahl S rats were greater than those in Dahl R rats on a high salt diet. The antihypertensive drugs, either nicardipine or captopril were administered to the Dahl S rats for 2 weeks beginning at 2 weeks after the start of the high salt diet. The nNOS mRNA in the PVN and SON of Dahl S rats given a high salt diet was not upregulated by treatment with nicardipine, while the nNOS mRNA in salt-loaded Dahl S rats was greater upregulated by treatment with captopril to a greater than without the antihypertensive drug. These results suggest that NO may have various physiological roles in the neuroendocrine system.
一氧化氮被认为不仅在外周系统,而且在中枢神经系统中作为细胞间通讯的串扰因子发挥着重要作用。本研究的目的是研究其对神经内分泌系统的生理作用,该系统整合了自主神经功能和内分泌。膜片钳对大鼠视上核(SON)的体外记录显示,NO供体增加了抑制性突触后电流。大鼠双侧后爪皮下注射5%福尔马林或生理盐水进行伤害性刺激。大鼠室旁核(PVN)神经元NO合成酶(nNOS)基因在s.c.注射福尔马林后2和6 h的表达均较未注射和生理盐水组显著升高。剥夺食物2 d后,大鼠PVN和SON中nNOS基因表达下调。瘦素的集中管理防止了食物匮乏引起的下调。锂诱导尿崩症大鼠PVN和SON中nNOS mRNA和NADPH-diaphorase活性升高。高血压达尔S大鼠PVN和SON中nNOS mRNA水平和NADPH-diaphorase活性均高于高盐饮食达尔R大鼠。从高盐饮食开始后2周开始,给予达尔S大鼠2周的降压药物尼卡地平或卡托普利。高盐饮食组达尔S大鼠PVN和SON的nNOS mRNA未被硝地平上调,而高盐饮食组达尔S大鼠卡托普利组nNOS mRNA的上调幅度大于未加卡托普利组。这些结果提示NO可能在神经内分泌系统中具有多种生理作用。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ozaki M.et al.: "Preferential potentiation by nitric oxide of spontaneous inhibitory postsynaptic currents in rat supraoptic neurones."Journal of Neuroendocrinology. 12. 273-281 (2000)
Ozaki M.等人:“一氧化氮对大鼠视上神经元自发抑制性突触后电流的优先增强。”神经内分泌学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Serino R. et al.: "Increased levels of hypothalamic neuronal nitric oxide synthase and vasopressin in salt-loaded Pah1 rat"Autonomic Neuroscience : Basic and Clinical. (in press).
Serino R. 等人:“盐负荷 Pah1 大鼠下丘脑神经元一氧化氮合酶和加压素水平升高”自主神经科学:基础和临床。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Isse T et al.: "Effects of leptin on fasting-induced inhibition of neuronal nitric oxide syuthase mRNA in the parauentricular and supraoptic nuclei of rats"Brain Research. 846. 229-235 (1999)
Isse T 等人:“瘦素对大鼠心室旁和视上核中禁食诱导的神经元一氧化氮合成酶 mRNA 抑制的影响”大脑研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Isse T.et al.: "Effects of leptin on fasting-induced inhibition of neuronal nitric oxide synthase mRNA in the paraventricular and supraoptic nuclei of rats."Brain Research. 846. 229-235 (1999)
Isse T.等人:“瘦素对空腹诱导的大鼠室旁核和视上核神经元一氧化氮合酶 mRNA 抑制的影响。”大脑研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ozaki M. et al.: "Preferential potentiation by nitric oxide of spontaneous inhibitory postsynaptic currents in rat supraoptic neurones"Journal of Neuroendocrinology. 12. 273-281 (2000)
Ozaki M.等人:“一氧化氮对大鼠视上神经元自发抑制性突触后电流的优先增强”神经内分泌学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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UETA Yoichi其他文献
UETA Yoichi的其他文献
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Challenge to reveal a novel role of central vasopressin and oxytocin neurons by optogenetic approach
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23659127 - 财政年份:2011
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Grant-in-Aid for Challenging Exploratory Research
Novel functional linkages of drinking・feeding behavior and TRP channels: Clarification by the post-genome approach
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- 批准号:
22390044 - 财政年份:2010
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$ 2.3万 - 项目类别:
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Novel roles of neurohypophyseal hormones and biological active substances on brain function in genetic animal model
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The localization and physiological role of adrenomedullin and related-peptides in the central nervous system
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10218210 - 财政年份:2002
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14370026 - 财政年份:2002
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$ 2.3万 - 项目类别:
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