Interaction between telomerase and histone acetylation in gastrointestinal carcinomas

1.胃肠道癌中组蛋白乙酰化状态乙酰化组蛋白H4（低乙酰化状态）的表达减少分别在66%的胃癌、46%的胃腺瘤、78%的结直肠癌和30%的结直肠腺瘤中检测到。组蛋白去乙酰化酶抑制剂TSA可抑制胃癌细胞的生长并诱导凋亡，TSA可诱导p21、CBP、巴克和cyclin E的表达;去甲基化酶MBD 2或TSA可抑制CD 44和p16的表达，而抑制HDAC 1的表达。组蛋白乙酰化和DNA甲基化的相互作用可能通过调节增殖和转移相关基因的表达参与胃肠道肿瘤的发生.端粒酶和端粒DNA相互作用分子在胃肠道肿瘤中的表达 ...更多信息 omas.大多数胃癌和结直肠癌组织中端粒酶活性与端粒酶逆转录酶的表达水平呈正相关。在胃的一些腺瘤和肠上皮化生中也检测到TERT表达。在50-60%的胃癌中检测到TRF 1、TRF 2和端锚聚合酶的表达增加。TIN 2和hRAP 1在胃癌中的表达分别为30%和65%。MRE 11复合物由MRE 11、RAD 50和作为DNA修复酶的NBS 1组成。MRE 11、RAD 50和NBS 1在胃癌中的表达分别为65%、70%和80%.胃肠道肿瘤中组蛋白乙酰化与端粒酶的关系大多数胃肠道肿瘤及部分癌前病变组织中组蛋白乙酰化水平低，端粒酶活性强，端粒酶活性表达增强，提示二者的相互作用可能在早期胃肠道肿瘤的发生中起重要作用。少

1. Histone acetylation status in gastrointestinal carcinomasReduced expression of acetylated histone H4 (low acetylation status) was detected in 66% of gastric cancers, 46% of gastric adenomas, 78% of colorectal cancers, and 30% of colorectal adenomas, respectively. Histone deacetylase inhibitor (trichostatin A : TSA) inhibited cell growth and induced apoptosis in gastric cancer cell lines, while TSA induced the expression of p21, CBP, BAK and cyclin E.Introduction of demethylase (MBD2) into gastric cancer cell line or TSA treatment retrieved the expression of CD44 and p16, while HDAC1 expression was suppressed. Interaction between histone acetylation and DNA methylation may be involved in gastrointestinal carcinogenesis through modulation of gene expression related to proliferation and apoptosis.2. Telomerase and telomere DNA interacting molecules in gastrointestinal carcinomasStrong telomerase activity and increased TERT expression were detected in most of the gastrointestinal carcin … More omas. The telomerase activities were well correlated with the levels of TERT expression in most of the gastric and colorectal carcinomas. TERT expression was also detected in some of the adenomas and intestinal metaplasia of the stomach. Increased expression of TRF1, TRF2 and tankyrase was detected in 50-60% of gastric carcinomas. The expression of TIN2 and hRAP1 was increased in 30% and 65% of gastric carcinomas, respectively. MRE11 complex consists of MRE11, RAD50 and NBS1 which acts as DNA repair enzyme. Increased expression of MRE11 RAD50 and NBS1 was found in 65%, 70% and 80% of gastric carcinomas, respectively.3. Relationship between histone acetylation and telomerase in gastrointestinal carcinomasMajorities of gastrointestinal carcinomas and a portion of precancerous lesions revealed low levels of histone acetylation and strong telomerase activity with increased TERT expression, suggesting that their interaction may deeply participate in gastrointestinal carcinogenesis at early stage. Less