Ribozyme approach to study functions of vascular endothelial growth factor isoforms on the progression of non-small cell lung cancer
核酶方法研究血管内皮生长因子亚型对非小细胞肺癌进展的作用
基本信息
- 批准号:11670227
- 负责人:
- 金额:$ 2.5万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The specific role of the cell-associated isoform of vascular endothelial growth factor (VEGF189) has not been clarified, while five isoforms of VEGF which have different biological activities (VEGF121, VEGF145, VEGF165, VEGF189, VEGF206) are generated by alternative splicing. We used a hammerhead-type ribozyme (V189Rz) to suppress VEGF189 mRNA.The V189Rz specifically cleaved exon 6 of VEGF189 mRNA, but showed no activity against VEGF121 or VEGF165 isoforms. The V189Rz was introduced into the human non-small cell lung cancer cell line (OZ-6/VR). The expression level of VEGF189 mRNA was decreased in the OZ-6/VR, while VEGF121 and 165 expression were preserved. The OZ-6/VR xenotransplanted into nude mice showed markedly reduced vascularisation and growth, whereas the cell line did not show any decreased growth under tissue culture conditions. The OZ-6/VR cells (1×10^5/mouse) formed no tumours, whereas the parental OZ-6 cells formed huge tumours within 8 weeks. The specific suppression of VEGF189 by the ribozyme decreased vascularity and xenotranplantability of the lung cancer cell line. The cell-associated isoform of VEGF (VEGF189) might have key roles on stromal vascularisation and growth of non-small cell lung cancer xenografts in vivo.
血管内皮生长因子细胞相关亚型(VEGF 189)的具体作用尚未阐明,而具有不同生物活性的五种VEGF亚型(VEGF 121、VEGF 145、VEGF 165、VEGF 189、VEGF 206)是通过选择性剪接产生的。我们使用锤头型核酶(V189 Rz)抑制VEGF 189 mRNA,V189 Rz特异性切割VEGF 189 mRNA的外显子6,但对VEGF 121或VEGF 165亚型没有活性。将V189 Rz导入人非小细胞肺癌细胞系(OZ-6/VR)。在OZ-6/VR中VEGF 189 mRNA的表达水平降低,而VEGF 121和165的表达保持不变。异种移植到裸鼠中的OZ-6/VR显示出显著减少的血管形成和生长,而细胞系在组织培养条件下没有显示出任何生长减少。OZ-6/VR细胞(1×10^5/小鼠)没有形成肿瘤,而亲代OZ-6细胞在8周内形成了巨大的肿瘤。核酶特异性抑制VEGF 189可降低肺癌细胞系的血管分布和异种移植性。VEGF的细胞相关亚型(VEGF 189)可能在体内非小细胞肺癌异种移植物的间质血管化和生长中起关键作用。
项目成果
期刊论文数量(14)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Okamoto K, Oshika Y, Fukushima Y, Ohnishi Y, Tokunaga T, Tomii Y, Kijima H, Yamazaki H, Ueyama Y, Nakamura M.: "Xenografts of human solid tumors frequently express cellular-associated isoform of vascular endothelial growth factor (VEGF) 189."Oncology Repo
Okamoto K、Oshika Y、Fukushima Y、Ohnishi Y、Tokunaga T、Tomii Y、Kijima H、Yamazaki H、Ueyama Y、Nakamura M.:“人类实体瘤的异种移植物经常表达细胞相关的血管内皮生长因子亚型(VEGF)
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- 影响因子:0
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Tsuchida T, Kijima H, Oshika Y, Tokunaga T, Abe Y, Yamazaki H, Tamaoki N, Ueyama Y, Scanlon K J, Nakamura M: "Hammerhead ribozyme specifically inhibits mutant K-ras mRNA of human pancreatic cancer cells."Biochemical and Biophysical Research Communications
Tsuchida T、Kijima H、Oshika Y、Tokunaga T、Abe Y、Yamazaki H、Tamaoki N、Ueyama Y、Scanlon K J、Nakamura M:“锤头核酶特异性抑制人胰腺癌细胞的突变 K-ras mRNA。”生物化学和生物物理
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- 影响因子:0
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Hatanaka H., Abe Y., Kamiya T., Morino F., Nagata J., Tokunaga T., Oshika Y., Suemizu H., Kijima H., Tsuchida T., Yamazaki H., Inoue H., Nakamura M.and Ueyama Y.: "Clinical implications of interleukin (IL)-10 induced by non-small-cell lung cancer."Annals
Hatanaka H.、Abe Y.、Kamiya T.、Morino F.、Nagata J.、Tokunaga T.、Oshika Y.、Suemizu H.、Kijima H.、Tsuchida T.、Yamazaki H.、Inoue H.、Nakamura M
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- 影响因子:0
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Okamoto K, Oshika Y, Fukushima Y, Yoshimura M, Ohnishi Y, Tokunaga T, Hashimoto T, Hatanaka H, Tomii, Y, Yoshida Y, Miura S, Tsuchida T, Kijima H, Yamazaki H, Nakamura M, Ueyama Y.: "Inhibition of liver metastasis of colon cancer by in vivo administration
冈本 K、Oshika Y、福岛 Y、吉村 M、Ohnishi Y、德永 T、桥本 T、Hatanaka H、Tomii、Y、吉田 Y、三浦 S、土田 T、木岛 H、山崎 H、中村 M、上山 Y:
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- 影响因子:0
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Tokunaga T, Nakamura M, Oshika Y, Abe Y, Ozeki Y, Fukushima Y, Hatanaka H, Sadahiro S, Kijima H, Tsuchida T, Yamazaki H, Tamaoki N, Ueyama Y.: "Thrombospondin 2 expression is correlated with inhibition of angiogenesis and metastasis of colon cancer."Briti
Tokunaga T, Nakamura M, Oshika Y, Abe Y, Ozeki Y, Fukushima Y, Hatanaka H, Sadahiro S, Kijima H, Tsuchida T, Yamazaki H, Tamaoki N, Ueyama Y.:“血小板反应蛋白 2 表达与血管生成抑制相关
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NAKAMURA Masato其他文献
ブラジルにおける燃料工タノ-ルの生産・利用の現状と評価(LCA手法によるサトウキビからの燃料エタノ-ルのwell-to-Wheel評価)
巴西燃料乙醇生产利用现状及评价(利用LCA方法对甘蔗燃料乙醇进行井到轮评价)
- DOI:
- 发表时间:
2006 - 期刊:
- 影响因子:0
- 作者:
YUYAMA Yoshito;1KUMURA Takashi;OHARA Akihiko;KOBAYASHI Hisashi;NAKAMURA Masato;小林 久 - 通讯作者:
小林 久
Evaluation of Performance and Costs of Various Biomass Conversion Technologies
各种生物质转化技术的性能和成本评估
- DOI:
- 发表时间:
2006 - 期刊:
- 影响因子:0
- 作者:
YUYAMA Yoshito;1KUMURA Takashi;OHARA Akihiko;KOBAYASHI Hisashi;NAKAMURA Masato - 通讯作者:
NAKAMURA Masato
EVALUATION OF REPAIR TIME BY AUTOMATIC GENERATION OF LOGIC NETWORK OF REPAIR SCHEDULE
自动生成维修计划逻辑网络评估维修时间
- DOI:
10.3130/aijs.85.1233 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
OKANO Hajime;NAGAYA Kazushi;NAKAMURA Masato;HASEGAWA Kan;KUSAKA Akihiro - 通讯作者:
KUSAKA Akihiro
NAKAMURA Masato的其他文献
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{{ truncateString('NAKAMURA Masato', 18)}}的其他基金
Basic Studies on the Provisions of "Non-intentional Murder" in Tang China
唐代“非故意杀人”规定基础研究
- 批准号:
20K01254 - 财政年份:2020
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Multidimensional Analysis of Cancer Stem Cell - Microenvironment Interaction with Patient Derived Xenografts (PDX) or Cancer Xenopatients
癌症干细胞的多维分析 - 微环境与患者来源的异种移植物 (PDX) 或癌症异种患者的相互作用
- 批准号:
15H04287 - 财政年份:2015
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Basic Study on the Pre-modern Chinese Judicial System, mainly in Tang Dynasty
中国近代司法制度基础研究(以唐代为主)
- 批准号:
22530004 - 财政年份:2010
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A Demonstrative Study on the Characteristics of Qing Legal System through Considering the Mitigatory Provisions in Qing Criminal Code
从清代刑法中的减刑规定看清代法律制度的特征实证研究
- 批准号:
17530007 - 财政年份:2005
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Observational study of Venus atmosphere dynamics
金星大气动力学观测研究
- 批准号:
17340147 - 财政年份:2005
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of infrared imaging devices for the observation of planetary atmosphere
开发用于观测行星大气的红外成像装置
- 批准号:
13440142 - 财政年份:2001
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Biopsychological research on the role of brain acetylcholine in intracranial self-stimulation behavior
脑乙酰胆碱在颅内自刺激行为中作用的生物心理学研究
- 批准号:
11610074 - 财政年份:1999
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of EUV optics for Oxygen ion imagery in the polar wind
用于极风中氧离子图像的 EUV 光学器件的开发
- 批准号:
10440135 - 财政年份:1998
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Intracellular localization of multidrug resistance associated protein (MRP) and atypical multidrug resistance phenomenon in non-small cell lung cancer
非小细胞肺癌多药耐药相关蛋白(MRP)的细胞内定位及非典型多药耐药现象
- 批准号:
09670205 - 财政年份:1997
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of EUV telescope for planetary plasma imgery
开发用于行星等离子体成像的 EUV 望远镜
- 批准号:
06452088 - 财政年份:1994
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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