Analysis of micrometastasis formation using marker geno-tagged tumor cells and its clinical application

标记基因标记肿瘤细胞微转移形成分析及其临床应用

基本信息

批准号：
11670236
负责人：
NAKANISHI Hayao
金额：
$ 2.3万
依托单位：
Aichi Cancer Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670236/
关键词：
GFP gene real-time analysis Stomach Cancer hematogenous metastasis peritoneal metastasis micro metastasis integrin マーカー遺伝子 GFP リアルタイムPCR 腹腔洗浄細胞診転移モデルルアルタイムPCR

项目摘要

1. We established GFP-expressing metastatic and non-metastatic rat tongue carcinoma cell lines that permits the real-time analysis of micrometastasis formation in combination with intravital video microscopy (IVVM) and demonstrated that micrometastasis formation in the liver consists of size-constraint initial tumor cell arrest, stable attachment to subsinusoidal BM and subsequent intravascular growth before extravasation.2. We established 3 peritoneal micrometastasis models including human gastric and colon cancercell lines tagged with GFP and found thatthere are two types ofperitoneal metastases. One is a omental metastasis type, in which tumorcells initially arrestonly in theomentumandtheotheris a peritoneal type, which form micrometastasis both in the ornentum and abdominal wall peritoneum at initial stage. In vitro analysis indicates that cytokines such as IL-1 beta, TNF-alpha may be responsible for peritoneal type metastasis via retraction of mesothelial lining.3. We established a real-time RT-PCR method forquantification of CEA mRNA in the peritoneal washes of gastric cancer patients and demonstrated that this method is a sensitive, quantitative, specific and rapid method to detect free cancer cells in peritoneal washes. We revealed apositive correlation between CEA mRNA levels in peritoneal washes and patient prognosis, indicating its usefulness to evaluate the risk ofperitoneal recurrence in patients with gastric cancer.

1. We established GFP-expressing metastatic and non-metastatic rat tongue carcinoma cell lines that permits the real-time analysis of micrometastasis formation in combination with intravital video microscopy (IVVM) and demonstrated that micrometastasis formation in the liver consists of size-constraint initial tumor cell arrest, stable attachment to subsinusoidal BM and subsequent intravascular growth before外部。2。我们建立了3个腹膜微量症模型，包括用GFP标记的人类胃和结肠抑制线，发现这是两种类型的螺旋转移。一种是胶质转移类型，其中肿瘤最初是在theomentumentumantheotheris theotheris类型中逮捕的，在初始阶段，在Ornentum和腹壁腹膜中形成微量含量。体外分析表明，诸如IL-1β，TNF-α之类的细胞因子可能通过缩回间皮内膜来负责腹膜类型转移。3。我们建立了一种实时的RT-PCR方法，用于在胃癌患者的腹膜洗涤中量化CEA mRNA，并证明该方法是一种敏感，定量，特定和快速的方法，可检测腹膜洗涤中的游离癌细胞。我们揭示了腹膜洗涤和患者预后中CEA mRNA水平之间的APOSISTIS相关性，这表明其有用性评估胃癌患者的腹膜复发风险。