DEVELOPMENT OF DNA VACCINES WHICH INDUCE DELAYED-TYPE CELLULAR IMMUNITY

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Listeria epitope-specific helper T cells (Th) were successfully primed in vivo by DNA immunization with a plasmid carrying class II-associated invariant chain (Ii) peptide (CLIP)-replaced murine Ii molecule. Immunizations by gene gun bombardment with plasmids carrying Ii molecules, whose CLIP regions were replaced with a Listeria-specific Th epitope, listeriolysin O (LLO) 215-226 (H2-E^k-restricted) or p60 301-312 (H2-A^d-restricted), was capable of priming specific Th showing proliferative responses to respective peptides in both C3H/He (H-2^k) and BALB/c (H-2^d) mice. Although the immunization to C3H/He mice induced specific interferon-γ-producing splenocytes and conferred significant protective immunity against listerial infection, the immunized BALB/c mice failed to do so. These results indicate that our CLIP-replaced Ii expression DNA vaccines are able to prime specific Th with different effector functions.