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Study of glial-neural response to ischemic and/or the other stress of the brain in forensic pathology

法医病理学中神经胶质神经对大脑缺血和/或其他应激反应的研究

基本信息

批准号：
11670420
负责人：
ORIHARA Yoshiyuki
金额：
$ 2.24万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670420/
关键词：
Forensic pathology Brain Glial-neural response Ischemic and or the other stress Immunohistochemistory Inducible nitric oxide synthase Apolipoprotein E 外傷性脳損傷アポリポプロテインE ニューロンアストロサイトミクログリア好中球血管平滑筋細胞

项目摘要

The aim of this study is to pursue the glial- neural response to ischemic and/or the other stress of the brain. Thus we investigated the dynamic induction / expression of inducible nitric oxide synthase (iNOS) and apolipoprotein E (apoE) using the human brains those causes of deaths were traumatized brain injuries. The localization of iNOS and apoE was evaluated by the technique of immunohistochemistry.Firstly, iNOS was not identified in the brains those survival time were within two days. Then, the immunoreactivity of iNOS was localized in the following regions, i.e. neutrophils and microglia/macrophages in the surrounding portions of necrosis on the traumatized cortical hemisphere, deep part of cortex and dentate gyri of hippocampi adjacent to hemorrhage, and within cytoplasm of vascular smooth muscle of small artery or arteriole surrounded to injuries in the brains those survival time were more than two days. These immunoreactivities of iNOS were not identified on and after eight da … More ys survival.Secondly, the striking staining difference was observed regarding to the expression of apoE between the traumatized cortical hemisphere and the contralateral cortical hemisphere to the injury. The apoE was identified within the neuron in traumatized hemisphere from very early period since injury. To the contrary, there was no positive apoE staining in the contralateral cortical hemisphere at all. The positive apoE staining within the neuron in the traumatized cortical hemisphere was gradually decreased regarding to both staining density and the cell count subsequently to ten days survival. Although the positive apoE staining within astrocytes was faint in the both traumatized and contralateral hemispheres at very early period after injury, intensive expression of apoE within the astrocytes in the traumatized hemisphere was observed at the short to long survival cases, for the instance, in even more than one month survival. Further, there was no apoE expression localized to microglia in any cases.These observations corroborated the prolonged induction within various cells of iNOS and apoE in the injured brain. These responses indicated that both iNOS and apoE play a crucial role to cerebrovascular damage and/or secondarily brain damage subsequent to brain injury. Less

本研究的目的是探讨神经胶质对脑缺血和/或其他应激的反应。因此，我们研究了诱导型一氧化氮合酶（iNOS）和载脂蛋白E （apoE）在脑外伤死亡人群中的动态诱导表达。免疫组化技术检测iNOS和apoE的定位。首先，存活时间在2天以内的小鼠大脑中未发现iNOS。然后，iNOS的免疫反应性定位于以下区域：创伤后皮层半球坏死周围的中性粒细胞和小胶质细胞/巨噬细胞，邻近出血的海马皮层深部和齿状回，以及被损伤包围的小动脉或小动脉血管平滑肌细胞质内，存活时间超过2天。这些免疫反应在8 d时和8 d后未被发现。其次，apoE在损伤皮质半球和对侧皮质半球的表达有显著的染色差异。apoE在损伤后很早就存在于损伤半球的神经元中。相反，对侧皮质半球未见apoE阳性染色。损伤后10天，皮层半球神经元内apoE阳性染色随染色密度和细胞计数逐渐减少。虽然损伤后极早期损伤半球和对侧半球星形胶质细胞内apoE阳性染色微弱，但在短至长存活的病例中，甚至存活1个月以上，损伤半球星形胶质细胞内apoE表达强烈。此外，在任何情况下，小胶质细胞中都没有apoE的表达。这些观察结果证实了损伤脑内各种细胞内iNOS和apoE的长期诱导。这些反应表明iNOS和apoE在脑损伤后的脑血管损伤和/或继发性脑损伤中起重要作用。少