IDENTIFICATION OF NITRIC OXIDE RELATED PROTEIN EXPRESSED IN TRANSACTIVATED ITO CELLS

反式激活 ITO 细胞中表达的一氧化氮相关蛋白的鉴定

• 批准号: 11670511
• 负责人: SAIBARA Toshiji
• 金额: $ 2.18万
• 依托单位: KOCHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670511/
• 关键词: ITO CELLS; LIVER; RAT; STELLATE CELLS; MYOFIBROBLAST; NITRIC OXIDE; rat; myofibroblast; nitric oxide; non-alcoholic steatohepatitis; NASH

Ito cells have a key role in regulating hepatic sinusoidal blood flow, because they contract in response to endothelin-1 and produce nitric oxide to dilate sinusoids. Progress in purification of Ito cells enabled us to obtain highly-purified cells and we investigate this process precisely through arginine transport and nitric oxide production in Ito cells. In this study, we developed monoclonal antibody for purifying protein transiently expressed in transformed Ito cells during the process of iNOS mediated nitric oxide production and partial amino acid sequence revealed it as unknown proteins. We need further investigation to recognize the role of these proteins in Ito cells during iNOS induction.Development of liver fibrosis is a life-threatening complications in chronic liver diseases. Oral administration of arginine, the substrate of nitric oxide, may be a promising way of an increase in sinusoidal blood flow in chronic liver diseases. A new insight into the iNOS related proteins expressed in Ito cells may provide an important information for improving hepatic blood flow.

ITO细胞在调节肝窦血流方面起着关键作用，因为它们在内皮素-1的作用下收缩，并产生一氧化氮来扩张肝窦。Ito细胞纯化的进展使我们能够获得高纯度的细胞，我们正是通过Ito细胞的精氨酸转运和一氧化氮的产生来研究这一过程。在本研究中，我们研制了用于纯化iNOS介导的一氧化氮产生过程中转化的ITO细胞中瞬时表达的蛋白的单抗，部分氨基酸序列表明它是未知的蛋白。我们需要进一步的研究来认识这些蛋白在诱导型一氧化氮合酶诱导过程中在ITO细胞中的作用。肝纤维化的发展是慢性肝病的一种威胁生命的并发症。口服精氨酸，一氧化氮的底物，可能是一种在慢性肝病中增加正弦血流量的有希望的方法。对iNOS相关蛋白在ITO细胞中表达的新认识可能为改善肝脏血流提供重要信息。

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