Development of noninvasive procedure for diagnosis of NASH and of therapeutic strategy

开发非侵入性 NASH 诊断方法和治疗策略

• 批准号: 13670524
• 负责人: SAIBARA Toshiji
• 金额: $ 2.18万
• 依托单位: Kochi medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670524/
• 关键词: STEATOHEPATITIS; COMPUTED TOMOGRAPHY; FERRITIN; PLATELET; AGE; aromatase; ArKO; cyp19

The pathological features of NASH are associated with the pathological features most commonly seen in alcoholic liver disease itself. However, liver biopsy is a surgical procedure and a serious complication is inevitable at a very low rate. In this study, we investigated to find a noninvasive procedure for a diagnosis of NASH. A multivariated analysis revealed that patients with severe hepatic steatosis defined by using liver/spleen ratio<0 and age>50 years old were two most important factors. Plasma ferritin and platelet number were also useful markers of NASH. Venosection was a useful strategy for NASH with elevated ferritin levels. Randomized therapeutic trial using metformin and PPAR-γ ligand is in process.

NASH的病理学特征与酒精性肝病本身最常见的病理学特征相关。然而，肝活检是一种外科手术，严重的并发症是不可避免的，发生率很低。在这项研究中，我们研究了寻找一种诊断NASH的非侵入性方法。多因素分析显示，50岁以上的重度肝脂肪变性患者（以肝/脾比值定义<0 and age>）是两个最重要的因素。血浆铁蛋白和血小板数量也是NASH的有用标志物。静脉切开术是治疗铁蛋白水平升高的NASH的有效策略。使用二甲双胍和PPAR-γ配体的随机治疗试验正在进行中。

项目成果

K.Toda, et al.: "Alterations in fatty-acid metabolism in the liver of ArKO male mice and their ameliorations by administration of 17 β-estradiol or a ligand of peroxisome proliferator-activated receptor α."J Steroid Biochem Mol Biol.. 79. 11-17 (2002)

K. Toda 等人：“ArKO 雄性小鼠肝脏中脂肪酸代谢的改变以及通过施用 17 β-雌二醇或过氧化物酶体增殖物激活受体 α 配体的改善。”J Steroid Biochem Mol Biol。 79. 11-17 (2002)

C-L Li, et al.: "Estrogen deficiency results in enhanced expression of Smoothened of the Hedgehog signaling in the thymus and affects thymocyte development."Intern Immunopharmacol. 2. 823-833 (2002)

C-L Li 等人：“雌激素缺乏会导致胸腺中 Hedgehog 信号的 Smoothened 表达增强，并影响胸腺细胞的发育。”Intern Immunopharmacol。

G-L Li, et al.: "Estrogen deficiency results in enhanced expression of Smoothened of the Hedgehog signaling in the thymus and affects thymocyte development"Intern immunopharmacol. 2. 823-833 (2002)

G-L Li 等人：“雌激素缺乏会导致胸腺中 Hedgehog 信号的 Smoothened 表达增强，并影响胸腺细胞发育”Internimmunopharmacol。

Wakatsuki A, et al.: "Size and oxidative susceptibility of low-density lipoprotein particles in breast cancer patients with tamoxifen-induced fatty liver"J Clin Endocrinol Metab. 87. 3676-3681 (2002)

Wakatsuki A 等人：“患有他莫昔芬诱导脂肪肝的乳腺癌患者中低密度脂蛋白颗粒的大小和氧化敏感性”J Clin Endocrinol Metab。

T. Saibara, et al.: "Effects of ethanol on 1-arginine transport in rat ito cells in relation to nitric oxide production"Alcohol Clin Exp Res.. 25. 39S-45S (2001)

T. Saibara 等人：“乙醇对大鼠细胞中与一氧化氮产生相关的 1-精氨酸转运的影响”Alcohol Clin Exp Res.. 25. 39S-45S (2001)