IDENTIFICATION OF NUCLEAR PROTEIN EXPRESSED IN TRANSACTIVATED ITO CELLS

肝纤维化的发展导致肝功能衰竭、肝细胞癌、食管静脉曲张和自发性腹膜炎，这些都是肝硬化危及生命的并发症。诱导转化的Ito细胞凋亡可能是治疗慢性肝病的一种有前景的方法。对转化过程中核蛋白表达变化的新认识可能为肝纤维化的治疗提供重要信息。伊藤细胞纯化的进展使我们获得了高纯度的细胞，我们精确地研究了转化过程。本研究制备了纯化转化Ito细胞中瞬时表达的核蛋白的单克隆抗体，氨基酸序列分析显示其为肌细胞中常见的emelin。在Ito细胞转化过程中，我们需要进一步研究emelin的瞬时表达。

Development of liver fibrosis results in hepatic failure, hepatocellular carcinoma, esophageal varices, and spontaneous peritonitis, which are life-threatening complications in liver cirrhosis. Induction of apoptosis in transformed Ito cells maybe a promising way of treatment of chronic liver disease. A new insight into the changes of nuclear protein expressed in the process of transformation may provide an important information for the treatment of liver fibrosis.Progress in purification of Ito cells enabled us to obtain highly-purified cells and we investigate the process of transformation precisely. In this study, we developed monoclonal antibody for purifying nuclear protein transiently expressed in transformed Ito cells during the process of transformation and the analysis of amino acid sequence revealed it as emelin commonly expressed in myocytes. We need further investigation to recognize the role of transient expression of emelin in Ito cells during transformation.