Increased fatty acid oxidation in immature reperfused hearts -Effects of insulin and glucagon-

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Animal : Japanese white rabbit, two-week-oldIsolated working heart perfusion : 2O min normoxia-40 min ischemia-40 min reperfusionPerfusion buffer : Krebs-Henselit (pH=7.4), including 1.25mM Ca^<2+>, 5.5mM glucose, 1.2mM ^<13>C-palmitate, 1.25mM lactate, 0.5mM acetoacetate.Indication of cardiac function : heart rate x systolic pressure (double product, DP), cardiac output x systolic pressure (cardiac work, CW)Myocardial ^<13>C-acetyl carnitine detection : Tandem massMyocardial CoA esters concentration : HPLCGroup : group 1 - no ischemia (40 min), insulin (40 U/ml) group 2 - no ischemia (40 min), glucagon (1 μg/ml) group 3 - reperfusion, insulin (40 U/ml) group 4 - reperfusion, glucagon (1 μg/ml)RESULTS :cardiac function (DP, CW) no ischemia : group 1 = group 2 reperfusion : group 3>group 4palmitate oxidation no ischemia : group 1<group 2 reperfusion : group 3<group 4tissue malonyl-CoA no ischemia : group 1>group 2 reperfusion : group 3>group 4In reperfused immature hearts, glucagon may have detrimental effects on the recovery of contracrail function due to accelerate fatty acid oxidation.