The Treatment of Atopic Dermatitis with PUVA Photochemotherapy : Clinical and Experimental Studies in Patients and Model Mice

PUVA 光化疗治疗特应性皮炎：患者和模型小鼠的临床和实验研究

• 批准号: 11670854
• 负责人: HORIO Takeshi
• 金额: $ 2.11万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670854/
• 关键词: atopic dermatitis; ultraviolet therapy; PUVA therapy; NC; Nga mouse; 光化学療法

Recently, the number of adult patients with severe atopic dermatitis is increasing in our clinic. For more than 10 years, we have treated these patients with oral 8-methoxypsoralen and whole body exposures to UVA (systemic PUVA therapy). In the present study, we surveyed the treated patients in order to establish the systemic PUVA therapy in Japanese adult patients with severe atopic patients. Patients were hospitalized, and given orally 30mg of 8-MOP and subsequently irradiated with 2〜6J/cm^2-UVA three times a week. Most of patients received 10 to 30 time-PUVA therapy and approximately 100 J/cm^2 of UVA in total. Severity scores of atopic dermatitis linearly decreased until 12 treatments, and reached plateau thereafter. No adverse effects occurred. In next experiments, we examined the therapeutic effects of PUVA treatment on the spontaneously developed dermatitis in NC/Nga model mice of atopic dermatitis. Photochemotherapy was done 3 times a week for 4 weeks. Skin changes began to improve by 3 treatments, and did not recur even 4 weeks after discontinuation of PUVA therapy. Collectively, systemic PUVA therapy seems to be a useful modality for the treatment of severe atopic dermatitis.

最近，我们诊所患有严重特应性皮炎的成人患者数量不断增加。十多年来，我们一直通过口服 8-甲氧基补骨脂素和全身 UVA 照射（全身 PUVA 疗法）来治疗这些患者。在本研究中，我们对接受治疗的患者进行了调查，以便在患有严重特应性患者的日本成人患者中建立全身性 PUVA 疗法。患者住院，口服30mg 8-MOP，随后每周3〜6J/cm^2-UVA照射3次。大多数患者接受了 10 至 30 次 PUVA 治疗，总计约 100 J/cm^2 的 UVA。特应性皮炎的严重程度评分线性下降，直到 12 次治疗后才达到稳定水平。没有发生不良反应。在接下来的实验中，我们检查了 PUVA 治疗对特应性皮炎 NC/Nga 模型小鼠自发性皮炎的治疗效果。光化疗每周进行3次，持续4周。经过3次治疗，皮肤变化开始改善，甚至在停止PUVA治疗4周后也没有复发。总的来说，全身性 PUVA 治疗似乎是治疗严重特应性皮炎的有效方法。

项目成果

Okamoto H, et al.: "Evaluation of apoptotic cells induced by ultraviolet light B radiation in epidermal sheets stained by the TUNEL technique."Journal of Investigative Dermatology. 113. 802-807 (1999)

Okamoto H 等人：“通过 TUNEL 技术染色的表皮片中紫外线 B 辐射诱导的凋亡细胞的评估。”研究皮肤病学杂志。

堀尾武: "生物の光障害とその防御機構"共立出版. 15 (2000)

Takeshi Horio：“生物体中的光损伤及其防御机制”Kyoritsu Shuppan 15 (2000)。

堀尾武: "今日の治療指針,1999"医学書院. 1 (1999)

Takeshi Horio：“当今的治疗指南，1999” Igaku Shoin 1 (1999)。

Yoshimura-Mishima M, et al.: "Suppressive effect of ultraviolet (UVB and PUVA) radiation on superantigen production by staphylococcus aureus."Journal of Dermatological Science. 19. 31-36 (1999)

Yoshimura-Mishima M 等人：“紫外线（UVB 和 PUVA）辐射对金黄色葡萄球菌产生超抗原的抑制作用。”皮肤病学杂志。

Miyauchi-Hashimoto H, et al.: "Ultraviolet radiation-induced suppression of natural killer cell activity is enhanced in xeroderma pigmentosum group A (XPA) model mice."Journal of Investigative Dermatology. 112. 965-970 (1999)

Miyauchi-Hashimoto H 等人：“在着色性干皮病 A 组 (XPA) 模型小鼠中，紫外线辐射诱导的自然杀伤细胞活性抑制得到增强。”皮肤病学研究杂志。