TO FIND THE WAY TO OVERCOME THE TUMOR ACCELERATED PROLIFERATION DURING FRACTIONATIONS OF RADIATION THERAPY

寻找克服放射治疗分次期间肿瘤加速增殖的方法

• 批准号: 11670858
• 负责人: ABE Yoshinao
• 金额: $ 1.92万
• 依托单位: HIROSAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670858/
• 关键词: RADIATION THERAPY; FRACTIONATION; ACCELERATED PROLIFERATION; CARBON ION BEAM; HIGH LET RADIOTHERAPY; JEJUNAL CRYPT; REPOPULATION; accelerated proliferation; accelerated proliferantion; 重イオン線; 高LET; 炭素線; 小腸コロニー法; 多分割照射; RBE

The tumor repopulation (accelerated proliferation) during fractionation is thought to be a factor causing insufficient radiation effects. Using low LET irradiation and mouse jejunal crypts, we studied the mechanism of accelerated proliferation. To summarize the previous results as follows ; 1) accelerated proliferation is observed during multiple fractions of 2 Gy dose, 2) the extent of proliferation depend on the total dose per a day and 3) there are 2 possible mechanisms for accelerated proliferation-shortening of potential doubling time and reduction of cell loss factor.The purpose of this study is to clarify whether accelerated proliferation found in jejunal crypt is observed during multiple doses of high LET beams such as carbon ion beam. Carbon ion beam of LET 70 keV/μm (290 MeV/u and 6 cm SOBP) was used. Endpoint was Jejunal colony assay. After obtaining RBE, fractionation effects were compared between X-ray irradiation and carbon ion beam. Experiments were performed as follows ; 1) a single dose, 2) two split dose at an interval of 4 h and 3) fractionation for 1 to 3 days with a single top-up dose. Fraction size of carbon ion beams and X-rays are 1 Gy and 2 Gy, respectively.The results showed colony survival obtained from carbon ion beam was shifted to the sensitive side compared with that obtained from X-rays. This is either due to incomplete repair or inhibition of accelerated proliferation. The former could be ruled out, since split dose study showed repair was completed between 4 h interval. From this study, it is concluded that fractionation of carbon ion beam could inhibit, in some extent, accelerated proliferation in jejunal crypt.Some other investigations about the human cancer were also studied.This study regarding the use of carbon ion beam is performed under a Research Project with Heavy Ions at NIRS-HIMAC.

分割过程中的肿瘤再增殖（加速增殖）被认为是导致辐射效应不足的一个因素。利用低LET照射和小鼠空肠隐窝，研究了其促增殖机制。将先前的结果总结如下：1）在2戈伊剂量的多个部分期间观察到加速的增殖，2）增殖的程度取决于每天的总剂量和3）加速增殖有2种可能的机制-本研究的目的是阐明在空肠隐窝中发现的加速增殖是否是在高LET束如碳离子束的多剂量期间观察到。采用LET 70 keV/μm（290 MeV/u和6 cm SOBP）的碳离子束。终点为空肠集落测定。在获得RBE后，比较了X射线辐照和碳离子束辐照的分馏效应。实验如下进行：1）单次给药，2）间隔4小时的两次分次给药，3）单次补充给药，分次给药1至3天。碳离子束和X射线的照射剂量分别为1戈伊和2戈伊，结果表明碳离子束照射的菌落存活率比X射线照射的菌落存活率向敏感侧移动。这是由于不完全修复或抑制加速增殖。前者可以排除，因为分次给药研究显示修复在4 h间隔之间完成。从这项研究中得出的结论是，碳离子束的分馏可以抑制，在一定程度上，在空肠隐窝的加速增殖。其他一些关于人类癌症的调查也进行了研究。这项研究是关于使用碳离子束的重离子在NIRS-HIMAC的研究项目下进行的。

项目成果

Y.Mariya 他: "Oxygenation status and tumor response during fractionated irradiation in two murine tumor cell lines of same origin but different intrinsic radiosensitivities"Radiat Med. 17・2. 175-179 (1999)

Y.Mariya 等人：“两种来源相同但内在放射敏感性不同的小鼠肿瘤细胞系中的氧合状态和肿瘤反应”Radiat Med 175-179 (1999)。

Y.Abe, Y.Mariya and M.Aoki: "Clinical and fundamental researches of radiotherapy."Hirosaki Med J. 51. s203-207 (1999)

Y.Abe、Y.Mariya 和 M.Aoki：“放射治疗的临床和基础研究”。Hirosaki Med J. 51. s203-207 (1999)

Y.Mariya 他: "Combined treatment effects of irradiation and SN-38, an active metabolite of camptothecin derivative irinotecan, on the human cell line PECA4197"Hirosaki Med J. 51・1. 10-14 (1999)

Y. Mariya 等：“放射线和喜树碱衍生物伊立替康的活性代谢物 SN-38 对人类细胞系 PECA4197 的组合治疗效果” Hirosaki Med J. 51·1 (1999)。

Y.Mariya 他: "Treatment outcome with T3NO-1 laryngeal carcinomas : Primary radiotherapy versus surgery with pre-/postoperative radiotherapy"Hirosaki Med J. 51・1. 38-43 (1999)

Y. Mariya 等：“T3NO-1 喉癌的治疗结果：初次放疗与术前/术后放疗的手术” Hirosaki Med J. 51・1 (1999)。

阿部 由直 他: "重粒子線照射による正常組織および腫瘍の増殖反応に関する研究"平成10年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書. 66-67 (1999)

Yunao Abe等：“重离子束照射对正常组织和肿瘤的生长反应的研究”1998年国立放射线科学研究所重离子束癌症治疗设备等的联合使用研究报告66-67（1999）