ANALYSIS OF RAT'S BEHAVIOR BY VIDEO AND BRAIN DOPAMIN METABOLISM DURING HYPERPHAGIA INDUCED BY SCHEDULED FEEDING

通过视频分析大鼠在定时喂食引起的暴食期间的行为和脑多巴胺代谢

• 批准号: 11670961
• 负责人: KIRIIKE Nobuo
• 金额: $ 1.98万
• 依托单位: OSAKA CITY UNIVERSITY MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670961/
• 关键词: restricted feeding; rebound hyperphagia; video monitoring system; rat; general behavior; eating behavior; animal model; eating disorders; video monitorinq system

In patients with eating disorder, not only abnormal feeding behavior but abnormal general behaviors like hyper activity, impulsive behavior are observed. To clarify the biological basis of these behavior, rats with restricted scheduled feeding (SF-group), food deprivation (DP-group), and control (C-group) were observed their general behaviors including eating behavior, moving time, moving length, and number of rearing using a video image motion analysis system. The extracellular concentrations of DA, DOPAC, and HVA were also measured in rat striatum using microdialysis technique. Before free feeding when they moved to the monitor cage which mean new environment, moving time and length were increased in SF-group but it last less than 60 min. After free feeding began, rats ate more food in the order of SF-, DP- and C-group due to their feeding paradigms. On the other hand, any other general behaviors analyzed did not differ. The results suggested that the meal size were changed between the paradigms. DA metabolism in the SF-group was increased after free feeding compared with other groups. As other general behaviors were not changed, the difference in the DA metabolism might be due to feeding related factors. DA metabolism did not differed in DP- and C-group in spite of the different food intake. These results further suggested that the increased DA metabolism was not due to feeding behavior itself, but was due to feeding paradigms like stress reaction. Even in human eating disorders, not only the change of eating pattern but the stress reaction accompany with the chronic diet might related to their neurotransmitter abnormalities.

在进食障碍患者中，不仅观察到异常进食行为，而且观察到异常的一般行为，如过度活跃、冲动行为。为了阐明这些行为的生物学基础，使用视频图像运动分析系统观察了限制性计划喂养（SF-组）、食物剥夺（DP-组）和对照（C-组）大鼠的一般行为，包括进食行为、移动时间、移动长度和直立次数。用微透析技术测定了纹状体细胞外DA、DOPAC和HVA的浓度。自由饲养开始后，SF-组大鼠进食量按SF-组、DP-组和C-组的顺序增加，而DP-组和C-组大鼠的进食量按SF-组、DP-组和C-组的顺序增加。另一方面，分析的任何其他一般行为没有差异。结果表明，膳食量的变化之间的范式。SF-组的DA代谢在自由喂养后比其他组增加。由于其他一般行为没有改变，DA代谢的差异可能是由于进食相关因素造成的。DA代谢在DP-和C-组中没有差异，尽管不同的食物摄入量。这些结果进一步表明，DA代谢增加不是由于摄食行为本身，而是由于摄食范式如应激反应。即使在人类进食障碍中，不仅进食方式的改变，而且伴随慢性饮食的应激反应也可能与其神经递质异常有关。

项目成果

Koki Inoue at al: "Video analvsis of rats' hyperphagic behavior induced by scheduled feeding"J Neuropsychiatry Clin Neurosci. 12.1. 137 (2000)

Koki Inoue 等人：“计划喂养引起的大鼠暴食行为的视频分析”J Neuropsychiatry Clin Neurosci。

Koki Inoue, Nobuo Kiriike, Sinichi Iwasaki, Katsuhito Hikiji, Masakage Okuno, Yasutoshi Fujisaki.: "Video analysis of rats' hyperphagic behavior induced by scheduled feeding"The Journal of Neuropsychiatry and Clinical Neurosciences. 12(1). 137 (2000)

Koki Inoue、Nobuo Kiriike、Sinichi Iwasaki、Katsuhito Hikiji、Masakage Okuno、Yasutoshi Fujisaki.：“计划喂养引起的大鼠摄食过多行为的视频分析”神经精神病学和临床神经科学杂志。

Koki Inoue et al: "Video analysis of rats' hyperphagic behavior induced by scheduled feeding"J Neuropsychiatry Clin Neurosci. 12(1). 137 (2000)

Koki Inoue 等人：“计划喂养引起的大鼠暴食行为的视频分析”J Neuropsychiatry Clin Neurosci。