Effects of maternal separation on high carbohydrate contained food consumption in rebound hyperphagia and neurotransmitter

母体分离对食量反弹和神经递质中高碳水化合物食物消耗的影响

• 批准号: 14570940
• 负责人: KIRIIKE Nobuo
• 金额: $ 1.73万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570940/
• 关键词: maternal separation; rat; feeding behavior; microdialysis; rebound hyperphagia; dopamine; serotonin; carbohydrate; 母子分離; 栄養素; 閉所ストレス; 制限給餌; ストレス; rebound hyperphagia; 心理ストレス

We studied effects of maternal separation and space restriction stress following time-restricted feeding on a selection of food contents and changes from baseline of neurotransmitters in the rats. Rats were maternally separated from their dams 6 hours per day from postnatal day to weaning day (at age of 21 days). Then, they fed freely ad libitum since at age of 9weeks, and they underwent space restriction stress following 2 hours time-restricted feeding for 6 days. Thereafter, they fed 4-kind food (standard food, carbohydrate-rich food, protein-rich food, fat-rich food) and were investigated changes from baseline of dopamine, serotonin and their metabolites by using microdialysis method. There was an interaction of food contents with space restriction stress. Only carbohydrate-rich food consumption per body weight was increased significantly by space restriction stress. Fat-rich food consumption was increased, but it was not significant. Changes of DA,DOPAC,HVA,5-HT,5-HIAA in the striatum by space restriction followed by free feeding were investigated by using microdialysis method. There were no significant changes in the levels of any neurotransmitters. These results suggest that increase of carbohydrate-rich food consumption induced by maternal separation and space restriction was not associated, with DA and 5-HT metabolism in the striatum.

我们研究了限时喂养后母亲分离和空间限制应激对大鼠食物选择和神经递质基线变化的影响。从出生日至断奶日（21日龄），每天将母鼠与母鼠分离6小时。9周龄开始自由采食，限时采食2小时后进行空间限制应激，连续6 d。随后，分别饲喂4种食物（标准食物、富含碳水化合物的食物、富含蛋白质的食物、富含脂肪的食物），并采用微透析法检测多巴胺、血清素及其代谢物的基线变化。食物含量与空间限制胁迫存在交互作用。空间限制胁迫只显著增加了单位体重富含碳水化合物的食物摄入量。高脂肪食物的摄入量增加了，但并不显著。采用微透析法观察限制空间后自由喂养纹状体中DA、DOPAC、HVA、5-HT、5-HIAA的变化。神经递质水平没有明显变化。这些结果表明，母亲分离和空间限制导致的高碳水化合物食物摄取量增加与纹状体中DA和5-羟色胺代谢无关。