Hepatocyte growth factor gene therapy of acute graft-versus-host disease

1.反复将人肝细胞生长因子(HGF)CDNA导入骨骼肌，抑制肠上皮细胞的凋亡和供体T细胞向肝脏的浸润，从而改善急性移植物抗宿主病(GVHD)引起的肠病和肝损伤。2.HGF基因转染抑制了肠道和肝脏中干扰素-γ和肿瘤坏死因子-α的表达，并降低了血清IL-12的水平。3.供体细胞的髓外造血能力增强，GVHD小鼠的存活率提高。4.HGF基因转染不影响供体T细胞对宿主抗原的反应(混合淋巴细胞反应，CTL活性)，从而促进GVHD小鼠的无白血病存活(HGF基因治疗分离了急性GVHD和移植物抗白血病效应)。5.HGF基因转染促进了造血干细胞来源的供者T细胞的免疫重建。

1.Repeated transfection of the human hepatocyte growth factor (HGF) CDNA into skeletal muscle inhibited apoptosis of intestinal epithelial cells and donor T-cell infiltration into the liver, thereby ameliorating the enteropathy and liver injury caused by acute graft-versus-host disease (GVHD).2.HGF gene transfection suppressed IFN-y and TNF-a expression the intestine and liver and decreased the serum IL-12.3.Extramedullary hematopoiesis by donor cells was increased, and the survival rate of GVHD mice was improved by HGF gene transfection.4.HGF gene transfection did not affect donor T cell responses to host antigens (mixed lymphocyte reaction, CTL activity), thereby promoting leukemia-free survival of GVHD mice (HGF gene therapy separated acute GVHD from graft-versus-leukemia effects).5.HGF gene transfection promoted immune reconstitution by hematopoietic stem cell-derived donor T cells.