Therapeutic effect of hematopoietic stem cell transplantation in combination with tissue regenerative factor on systemic sclerosis

造血干细胞移植联合组织再生因子治疗系统性硬化症的疗效观察

• 批准号: 15591071
• 负责人: IWASAKI Tsuyoshi
• 金额: $ 2.11万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591071/
• 关键词: systemic sclerosis; HGF; hematopoietc stem cell transplantation; gene therapy; HVJ-liposome; 骨髄移植; HVJリポゾーム

1)Transplantation of T-cell depleted bone marrow cells from BALB/c mice improved skin thickness by Tsk mice2)HGF gene transfection improved skin thickness by Tsk mice.3)Combination of bone marrow transplantation and HGF gene therapy significantly improved skin thickness by Tsk mice but did not improved pulmonary fibrosis.4)Transplantation of T-cell depleted bone marrow cells shifted immune responses from Th2 to Th1.5)Donor cells were engrafted at 2 weeks after transplantation, but were rejected at 4 weeks after transplantation.

1)移植BALB/c小鼠去t细胞骨髓细胞可改善Tsk小鼠皮肤厚度2)HGF基因转染可改善Tsk小鼠皮肤厚度。3)骨髓移植联合HGF基因治疗显著改善了Tsk小鼠的皮肤厚度，但没有改善肺纤维化。(4) t细胞枯竭骨髓细胞移植使免疫反应从Th2转移到Th1.5)供体细胞在移植后2周移植，但在移植后4周出现排斥反应。