Diagnosis and treatment of multicentric occurring hepatocellular carcinoma using molecular biological technique

分子生物学技术对多中心性肝细胞癌的诊治

• 批准号: 11671263
• 负责人: SHIMADA Hiroshi
• 金额: $ 2.11万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671263/
• 关键词: hepatocellular carcinoma; multicentricity; intrahepatic metastasis; survival rate; HBV-DNA integration pattern; nm23-H1 gene; p53 gene; nm23-H1遺伝子; TAI; p^<53>遺伝子

<Purpose>The aim of this study was to differentiate metachronously multicentric occurring hepatocellular carcinoma (MC) from intrahepatic metastatic HCC (IM) and to clarify clinical characteristics of them.<Materials and methods>Among 110 HCC patients with solitary nodules in the diameter of less than 5 cm having no capsular invasion and portal thrombus, 45 had recurrence. Recurrent HCC of twenty-nine patients were studied. Histological criteria and molecular biological criteria using HBV-DNA integration pattern, p53 mutation pattern and nm23-H1 expression were applied to define the multicentricity.<Results>1. MC was defined in 14 patients (61 %), IM in 9 (39 %) and unclassified cases was in 6. Among 23 patients 16 was defined histologically and 7 was molecular biologically.2. Solitary recurrence were found in 1 of 9 (11 %) IM patients and 11 of 14 (80 %) MC patients, respectively (p < 0.05), well-differentiated HCC were found in 0 of 9 (0 %) and 8 of 14 (57 %), respectively (p < 0.05). Interval time between the hepatectomy and recurrence were 13.5 months in IM and 26.7 months in MC, respectively (p < 0.05).<Conclusion>1. MC was defined in 14 patients (61 %) and IM in 9 (39 %).2. From the clinical viewpoint solitary well-differentiated HCC which was detected 2 years after the hepatectomy might be MC.

<ox>这项研究的目的是区分肝细胞质癌（MC）与肝内转移性HCC（IM）区分开，并澄清它们的临床特征。研究了29例患者的复发性HCC。使用HBV-DNA整合模式，p53突变模式和NM23-H1表达来定义多中心性。<结果> 1。在14例患者（61％）中定义了MC，在9例（39％）中进行了IM，而未分类的病例为6例。在23例患者中，有16例在组织学上定义了16例，7例是分子生物学上的。2。在9名IM患者中有1例中发现了孤立性复发，分别在14例（80％）MC患者中（p <0.05），分别在9（0％）中的0（0％）和14个（57％）中分别发现了良好的HCC（P <0.05）。肝切除术和复发之间的间隔时间分别为13.5个月，MC分别为26.7个月（p <0.05）。<结论> 1。在14例患者（61％）中定义了MC，在9例（39％）中定义了MC。从临床观点来看，在肝切除术后2年被检测到的孤立良好的HCC可能是MC。

项目成果

長堀 薫: "術中右肝動脈損傷時の対処"日本臨床外科学会雑誌. (in press). (2000)

Kaoru Nagahori：“手术期间右肝动脉受伤时的应对”，日本临床外科学会杂志（2000 年出版）。

Hitoshi Sekido: "Liver transplantation for primary sclerosing cholangitis"J Hepatobiliary Pancreat Surg. 6. 373-376 (1999)

Hitoshi Sekido：“原发性硬化性胆管炎的肝移植”J Hepatobiliary Pancreat Surg。

長堀 薫: "肝細胞癌と鑑別が困難であった肝細胞腺腫の1例"肝臓. 42(6). 321-326 (2001)

Kaoru Nagahori：“一例难以与肝细胞癌鉴别的肝细胞腺瘤” 肝脏 42(6) (2001)。

Y Nagano: "Laparoscopic surgery for pedunculated focal nodular hyperplasia, report of a case"Endoscopy. 31(Suppl1). 24-25 (1999)

Y Nagano：“腹腔镜手术治疗带蒂局灶性结节性增生，一例报告”内窥镜检查。

永野 靖彦: "膵頭十二指腸切除術にて切除しえた大腸癌肝転移切除後膵頭後面リンパ節再発の1例"日本臨床外科学会雑誌. (in press). (2000)

Yasuhiko Nagano：“通过胰十二指肠切除术切除结直肠癌肝转移后胰头后侧淋巴结复发的病例”日本临床外科学会杂志（2000 年出版）。