Analysis of chloroplast division mechanism by genome information, gene expression profile and reverse genetics.

通过基因组信息、基因表达谱和反向遗传学分析叶绿体分裂机制。

• 批准号: 18570036
• 负责人: SHIMADA Hiroshi
• 金额: $ 2.59万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570036/
• 关键词: Genome; Plant; Regulation of expression; Chloroplast

I selected 120 genes that are co-expressed with ARC3 and FtsZ genes, chloroplast division factor, using a gene expression profile in Arabidopsis thaliana, and I got the T-DNA line of the each gene from a stock center. I screened the homo lines of T-DNA insertion from the seeds by PCR. A number or size of chloroplasts in the homo line cells were observed with an optical microscope. The T-DNA lines of At1g56050 and At1g26230 had a small number of abnormally large chloroplasts in the cells, suggesting that At1g56050 and At1g26230 were the novel chloroplast division factors. The interaction of ARC3 protein and At1g56050 or At1g26230 were analyzed with yeast two-hybrid system. The results indicated that At1g56050 protein interacted with ARC3 protein. It was suggested that At1g56050 (GTP-binding protein) was the novel chloroplast division factor.DNA array analysis revealed that gene expression profile between wild type and arc3 mutant plants was almost same but the expression of At5g42825 in the mutant was higher than that of wild type. At5g42825 gene has no clear protein coding region, suggesting that At5g42825 might have a function as native RNAi.

我选择了与Arc3和FTSZ基因共表达的120个基因，即叶绿体分裂因子，使用拟南芥中的基因表达谱，然后我从股票中心获得了每个基因的T-DNA系。我通过PCR筛选了从种子中插入的T-DNA插入线。使用光学显微镜观察到同型线细胞中叶绿体的数量或大小。 AT1G56050和AT1G26230的T-DNA系在细胞中具有少量较大的叶绿体，这表明AT1G56050和AT1G26230是新型叶绿体分裂因子。用酵母双杂交系统分析了ARC3蛋白和AT1G56050或AT1G26230的相互作用。结果表明AT1G56050蛋白与ARC3蛋白相互作用。有人提出，AT1G56050（GTP结合蛋白）是新型的叶绿体分裂因子。DNA阵列分析表明，野生型和Arc3突变植物之间的基因表达谱几乎是相同的，但在突变体中AT5G42825的表达比野生型高。 AT5G42825基因没有明确的蛋白质编码区域，这表明AT5G42825可能具有天然RNAi的功能。

项目成果

Arabidopsis Cotyledon-Specific Chloroplast Biogenesis Factor CYOl is a Protein Disuflide Isomerase.

拟南芥子叶特异性叶绿体生物发生因子CYO1是蛋白质二硫键异构酶。

• 发表时间: 2007
• 期刊: The Plant Cell 19
• 作者: Hiroshi Shimada;et. al.
• 通讯作者: et. al.

Arabidopsis cotyledon-specific chloroplast biogenesis factor CYO1 is a protein disulfide isomerase

• DOI: 10.1105/tpc.107.051714
• 发表时间: 2007-10-01
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Shimada, Hiroshi;Mochizuki, Mariko;Takamiya, Ken-ichiro
• 通讯作者: Takamiya, Ken-ichiro

Identification of SIP that interacts with SPB, a trans-acting repressor, in Rhodobacter sphaeroides.

鉴定在球形红杆菌中与 SPB（一种反式作用阻遏蛋白）相互作用的 SIP。

• 发表时间: 2007
• 期刊: Plant&Cell Physiology 48
• 作者: Hiroshi Shimada;et. al.
• 通讯作者: et. al.

Identification of SIP that interacts with SPB, a trans-acting repressor, in Rhodo bacter sphacroides.

鉴定球形红细菌中与 SPB（一种反式作用阻遏蛋白）相互作用的 SIP。

• 发表时间: 2007
• 期刊: Plant & Cell Physiology 48
• 作者: Hiroshi Shimada;et. al.
• 通讯作者: et. al.

H., Ishida, K., Machiya, Y. and Takamiya, K. Isolation of SIP, a protein that interacts with SPB, a possible transcriptional regulatory factor in Rhodobacter sphaeroides.

H.、Ishida, K.、Machiya, Y. 和 Takamiya, K. SIP 的分离，SIP 是一种与 SPB 相互作用的蛋白质，SPB 是球形红杆菌中可能的转录调节因子。

• 发表时间: 2007
• 期刊: Plant &Cell Physiology 48
• 作者: H.;Shimada
• 通讯作者: Shimada