Experimental study of mature pulmonary lobar allo-transplantation in puppies.

幼犬成熟肺叶同种异体移植的实验研究。

• 批准号: 11671347
• 负责人: KAWAHARA Katsunobu
• 金额: $ 1.22万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671347/
• 关键词: lung transplantation in children; Pulmonary allo-transplantation; Pulmonary lobar transplantation; Living donar lobar transplantation

Back ground : The use of reduced-size adult lung transplantation could help solve the profound pediatric donor lung shortage. However, adequate long-term function of mature grafts requires growth in proportion to the recipient's development. Methods : Six mature left lower lobes from adult beagles (mean body weight : 12kg) were harvested and transplanted to six immature beagles (age : 3 months, mean body weight : 5kg) that underwent left pneumonectmy (ID). The control group (MD) was 5 adult dogs that underwent mature pulmonary lobar allotransplantation. Right pulmonary arterial occlusion test was performed under mechanical ventilation with Fi02 1 in 12 months or more after transplantation. Cardiac output and pulmonary arterial pressure (PAP) were measured, femoral arterial blood gas analysis (PaOa and PaCOa) was performed, and AaDO2 was calculated before and after occlusion at 5, 10, 15, and 30 minutes. The grafts were removed and examined histologically. Results : In ID, five dogs survived and three dogs tolerated the right pulmonary arterial occlusion test. In MD, three dogs survived and tolerated the occlusion test. There was no significant difference in mean PAP elevation between ID and MD. There were no differences in changes in Pa02, PaCO2 and AaDO before and 30 minutes after occlusion. Histological studies showed normal architecture of lung parenchyma but alveolar enlargement was observed in both ID and MD grafts.Conclusion : We conclude that in this experimental model of mature lobar transplantation in puppies, the alveolar architecture of a mature lobe is almost normal appearance or emphysema, and the long term function of a mature lobe is maintained well.

背景：减体积成人肺移植的使用可以帮助解决严重的儿科供肺短缺问题。然而，成熟移植物的足够的长期功能需要与受体发育成比例的生长。研究方法：取成年比格犬（平均体重12kg）左肺下叶6片，移植于未成年比格犬（年龄3月龄，平均体重5kg）左肺切除术（ID）后。对照组（MD）为5只成年犬，行同种异体成熟肺叶移植。移植后12个月或更长时间在Fi02 1的机械通气下进行右肺动脉闭塞试验。分别于封堵前、封堵后5、10、15、30 min测定心输出量、肺动脉压（PAP）、股动脉血气分析（PaOa、PaCOa），计算AaDO2。取出移植物并进行组织学检查。结果：ID组5只存活，3只耐受右肺动脉阻断试验。在MD中，3只犬存活并耐受闭塞试验。ID和MD之间的平均PAP升高无显著差异。阻塞前和阻塞后30分钟的Pa02、PaCO2和AaDO变化无差异。组织学研究表明，正常的肺实质结构，但肺泡扩大观察ID和MD grafts.Conclusion：我们得出结论，在这个实验模型的成熟肺叶移植幼犬，肺泡结构的成熟肺叶几乎是正常的外观或肺气肿，和长期功能的成熟肺叶保持良好。

项目成果

Kumagai K: "Obliterative airway diseaseand graft stenting in pig-to-dog tracheal xenotransplantation"The Japanese Journal of Thoracic and Cardiovascular Surgery. 49. 53-57 (2001)

Kumagai K：“猪对狗气管异种移植中的闭塞性气道疾病和移植物支架”《日本胸心血管外科杂志》。

Kawahara K: "Obliterative airway disease and graft stenting in pig-to-dog tracheal xenotransplantation"The Japanese Journal of Thoracic and Cardiovascular Surgery. 49. 53-57 (2001)

Kawahara K：“猪对狗气管异种移植中的闭塞性气道疾病和移植支架置入术”《日本胸心血管外科杂志》。

Kawahara K: "Obliterative airway disease and graft stenting in pig-to-dog tracheal xenotransplantation"The Japanese Journal of Thoracic and Cardiovascular Surgery. 49・1. 53-57 (2001)

Kawahara K：“猪-狗气管异种移植中的闭塞性气道疾病和移植支架”，《日本胸心血管外科杂志》49·1（2001）。