Experimental study on protection of pulmonary edema after lung and heart-lung transplantation
肺及心肺移植术后肺水肿保护作用的实验研究
基本信息
- 批准号:01570787
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1991
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Graft failure after lung or heart-lung transplantation is often caused by pulmonary edema resulting from ischemic reperfusion injury of the transplanted lung. It is well known that oxygen free radicals produced by neutrophils create reperfusion injury in iscliemic organs. We studied the prevelition of pulmonary reperfusion injury after canine lung and heart-lung allotransplantation.1. To evaluate the efficacy of antioxidants such as glutathione, ascorbic acid (vitamin C), alphatocopherol (vitamin E), superoxide dismutase (SOD), and CoQ10, the left lung was flushed with Euro-Collins solution containing one of these antioxidants, and then a hilar clamp was performed in this lung. Ascorbic acid and alpha-tocopherol were administered intravenously prior to declamping. In the lungs treated with antioxidants, the production of oxygen free radicals decreased, and oxygenation was comparable to that in untreated lungs. Therefore, infusion of these antioxidants may be useful for preventing pulmo … More nary edema after transplantation.2. Ischemic cold storage of canine left lungs at 4゚C for 24 hours was performed using a technique of flushing with preservative solution, and the lungs were reperfused with whole blood or leukocytedepleted blood. In the lungs flushed with Euro-Collins Solution, the lung water volume after reperfusion with leukocyte-depleted blood was lower than that with whole blood. On the other hand, in the lungs flushed with UW solution, which contained antioxidants such as glutathione and allopurinol, there was no difference between the lung water volume after reperfusion with whole vs leukocytedepleted blood. Results suggested that oxygen free radicals originating from neutrophils are one of the most important factors in the occurrence of ischemic reperfusion injury in grafted lungs, acid that perfusion with leukocyte-depleted blood prior to recirculation of the iscliemic lung is effective in preventing or alleviating pulmonary edema.3. We successfully allotransplaned canine lungs after cold strage for 8 hours during which the lungs were flushed and preserved with Euro-Collins solution containing antioxidants such as glutathione, allopurinal, and SOD. Furthermore, allotransplantation of canine lungs flushed acid preserved with UW solution at 4゚C for 24 hours was also successful. However, the safety limit of the cold preservation time of a canine heart-lung block for transplantation was 4 hours even when using the UW solution for flushing and preserving the graft.4. To evaluate the optimum potassium concentration in the UW solution, the lung water volume after reperfusion was evaluated using an isolated canine lung perfusion model. Canine left lungs were flushed with UW solution containing potassium at 120, 30, or 4 mEq/L, and preserved at 4゚C for 24 hours, then the lungs were reperfused with whole blood for 120 minutes. The lung water volume was lowest in the lungs flushed with UW solution containing potassium at 30 mEq/L, suggesting that this is the optimum potassium concentration in UW solution for preserving heart-lung grafts. Less
肺或心肺移植后移植物衰竭通常是由于移植肺缺血再灌注损伤引起的肺水肿引起的。众所周知,中性粒细胞产生的氧自由基在缺血器官中造成再灌注损伤。我们研究了犬肺和心肺同种异体移植后肺再灌注损伤的发生情况。为了评价抗氧化剂如谷胱甘肽、抗坏血酸(维生素C)、维生素E、超氧化物歧化酶(SOD)和辅酶Q10的功效,用含有这些抗氧化剂之一的Euro-Collins溶液冲洗左肺,然后在该肺中进行肺门钳夹。在开放夹闭前静脉给予抗坏血酸和α-生育酚。在用抗氧化剂处理的肺中,氧自由基的产生减少,氧合与未处理的肺相当。因此,输注这些抗氧化剂可能有助于预防肺动脉高压。 ...更多信息 移植后无水肿.采用保存液冲洗的方法,将犬左肺在4 ℃下缺血冷藏24小时,然后用全血或去白细胞血进行再灌注。在用Euro-Collins溶液冲洗的肺中,用去白细胞血液再灌注后的肺水量低于全血。另一方面,在用UW溶液(含有谷胱甘肽和别嘌呤醇等抗氧化剂)冲洗的肺中,全血与去白细胞血液再灌注后的肺水量没有差异。结果提示中性粒细胞产生的氧自由基是移植肺缺血再灌注损伤发生的重要因素之一,缺血肺再灌注前灌注去白细胞血可有效预防或减轻肺水肿.我们成功地进行了犬同种异体肺移植后,冷处理8小时,在此期间,肺冲洗和保存的Euro-Collins液含有抗氧化剂,如谷胱甘肽,别嘌呤和SOD。此外,用UW液在4 ℃保存24小时的犬肺同种异体移植也获得成功。但即使使用UW液冲洗和保存移植物,犬心肺块冷保存时间的安全限度也为4 h.为了评估UW溶液中的最佳钾浓度,使用离体犬肺灌注模型评估再灌注后的肺水量。用含钾120、30或4 mEq/L的UW溶液冲洗犬左肺,并在4 ℃下保存24小时,然后用全血再灌注肺120分钟。在用含30 mEq/L钾的UW溶液冲洗的肺中,肺水量最低,表明这是UW溶液中保存心肺移植物的最佳钾浓度。少
项目成果
期刊论文数量(52)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
碇 秀樹: "イヌ摘出左肺潅流モデルによる保存液の評価" 第6回肺および心肺移植研究会.
Hideki Ikari:“使用犬离体左肺灌注模型评估保存液”第6届肺和心肺移植研究组。
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K.Kawahara: "Graft function associated with oxygen free radicals immediately after transplantation" Acta Medica Nagasakinsia. 35. 225-227 (1990)
K.Kawahara:“移植后立即与氧自由基相关的移植功能”Acta Medica Nagasakinsia。
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Katsunobu Kawahara: "Twentyーfourーhour canine lung preservation using UW solution" Transplantation. 51. 584-587 (1991)
Katsunobu Kawahara:“使用 UW 溶液进行二十四小时犬肺保存”移植。 51. 584-587 (1991)
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小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,村田 謙,綾部 公懿,富田 正雄: "Low potassium UW液による24時間肺保存の実験的検討" 日本呼吸器外科学会雑誌. 5. 324 (1991)
Masahiro Kobayashi、Katsunobu Kawahara、Takao Takahashi、Shinji Akamine、Noriaki Itoyanagi、Ken Murata、Koki Ayabe、Masao Tomita:“使用低钾 UW 溶液进行 24 小时肺保存的实验研究”日本呼吸外科学会杂志 5。 324 (1991)
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村岡 昌司,川原 克信,高橋 孝郎,山本 聡,中村 昭博,田川 務,綾部 公懿,富田 正雄: "CMH solutionによる24時間冷却保存肺の機能に関する実験的検討" 第19回臓器保存研究会(H4.3.23).
Masashi Muraoka、Katsunobu Kawahara、Takao Takahashi、Satoshi Yamamoto、Akihiro Nakamura、Tsutomu Takawa、Koki Ayabe、Masao Tomita:“利用 CMH 溶液进行 24 小时冷藏肺功能的实验研究”第 19 期器官保存研究组(H4.3.23) )。
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KAWAHARA Katsunobu其他文献
KAWAHARA Katsunobu的其他文献
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{{ truncateString('KAWAHARA Katsunobu', 18)}}的其他基金
Effect of thoracoscopic right upper mediastinal dissection for left lung cancer-Especially about micrometastasis to lymph nodes-
胸腔镜右上纵隔切除术治疗左肺癌的效果-特别是淋巴结微转移-
- 批准号:
21591819 - 财政年份:2009
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Experimental study of mature pulmonary lobar allo-transplantation in puppies.
幼犬成熟肺叶同种异体移植的实验研究。
- 批准号:
11671347 - 财政年份:1999
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effect of cryopreservation on xenograft rejection following tracheal
冷冻保存对气管术后异种移植排斥反应的影响
- 批准号:
08671554 - 财政年份:1996
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Research Project on the Clinical Indication in Japan and the Posttransplant Patient Management in Lung and Heart-Lung Transplantation.
日本肺和心肺移植的临床适应症和移植后患者管理研究项目。
- 批准号:
09357013 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Co-operative Research on Clinical Application and Functional Preservation of Heart and Heart-lung Transplantation
心、心肺移植临床应用及功能保存合作研究
- 批准号:
07307015 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Research Project to Establish the Clinical Indication and the Way for Safe Management of the Graft in Lung and Heart-Lung Transplantation.
确定肺和心肺移植中移植物的临床适应症和安全管理方法的研究项目。
- 批准号:
06304036 - 财政年份:1994
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Basic Research of Heart Lung Transplantation
心肺移植基础研究
- 批准号:
02454325 - 财政年份:1990
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
CoーOperative Study for Clinical Application of Lung and Heart-Lung Transplantation
肺与心肺移植临床应用的合作研究
- 批准号:
01304043 - 财政年份:1989
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
Experimental investigation of heart-lung transplantation
心肺移植的实验研究
- 批准号:
62480292 - 财政年份:1987
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Multiple basic research for clinical application of the heart and heart-lung transplantation
心脏及心肺移植临床应用多项基础研究
- 批准号:
61480299 - 财政年份:1986
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Exterimental study on heart lung transplantation in monkey
猴心肺移植的实验研究
- 批准号:
61480298 - 财政年份:1986
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Lung and Heart-Lung Transplantation - Indication, Preservation and Retaining of Long-term Function.
肺和心肺移植——长期功能的适应症、保存和保留。
- 批准号:
60304069 - 财政年份:1985
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)