Effect of cryopreservation on xenograft rejection following tracheal

冷冻保存对气管术后异种移植排斥反应的影响

• 批准号: 08671554
• 负责人: KAWAHARA Katsunobu
• 金额: $ 1.34万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671554/
• 关键词: Tracheal xenotransplantation; Cryopreservation; Xenogeneic microchimerism; Obliterative airway disease; Tracheal stent; 気管冷凍保存; 気管異種移植; 冷凍保存; FK506; Deoxyspergualin

1. Pig-to-dog cryopreserved tracheal xenotransplantation.Pigtracheaswere harvested and stored at-8OﾟCfor2or4weeks.Inxenograftsrejection, obliterative airway disease (OAD) occurs very soon after transplantation in pig-to-dog discordanttracheal xenografts. Stenting with grafts is useful for the management of tracheal obstruction.2. Expression of CD4, CD8, and lgM in guinea pig-to-rat cryopreserved tracheal xenografts.OAD occurs in rodents with concordant xenografts. CDI4+cell and lgM+ cell infiltration was observed in all ofxenografts, while neither CD4+cell nor lgM+ cell infiltration was observed in syngeneicgrafts. This suggests that both cellular and humoral immunity causes OAD.Cryopreservation may notreduce xenograft rejection.3. Xenogeneic microchimerism in hamster-to-rat tracheal xenotransplantation.Xenogeneic microchimerisms were evaluated using polymerase chain reaction with primers specificfor the hamster hypoxanthine phosphoribosyl-transferase gene. Xenogeneic microchimerism was notfoundin the peripheral polymorphonulrear leukocytes of recipient rats for during the days post-transplantation.

1.猪-狗低温保存气管异种移植术：取猪气管，-80 ℃保存2或4周，猪-狗不协调气管异种移植术后，移植物排斥反应迅速，出现闭塞性气道疾病（OAD）。结论：1.支架植入术是治疗气管阻塞的有效方法.豚鼠-大鼠冷冻保存气管异种移植物中CD 4、CD 8和IgM的表达。OAD发生于具有一致异种移植物的啮齿动物。异种移植物中均可见CD 4+细胞和IgM+细胞浸润，而同系移植物中未见CD 4+细胞和IgM+细胞浸润。这表明细胞免疫和体液免疫都是导致OAD的原因，冷冻保存不能减少异种移植排斥反应.仓鼠-大鼠异种气管移植中的异种微嵌合体：用仓鼠次黄嘌呤磷酸核糖转移酶基因特异性引物进行聚合酶链反应，评价异种微嵌合体。在移植后的几天内，在受体大鼠的外周血多形核白细胞中未发现异种微嵌合体。

项目成果

Kawahara K,et al.: "Tracheal transplantation for carinal reconstruction in dogs" J.Thorac.Cardiovasc.Surg.116. 397-401 (1998)

Kawahara K 等人：“犬隆突重建的气管移植”J.Thorac.Cardiovasc.Surg.116。

Kawahara K, et al.: "Tracheal thansplantation for carinal reconstruction indogs" J.Thorac.Cardiovasc.Sarg.116. 397-401 (1998)

Kawahara K 等人：“犬隆突重建的气管移植术”J.Thorac.Cardiovasc.Sarg.116。

巻幡 聰、川原克信他: "異種気管移植の実験的検討" 呼吸器外科. 発表予定.

Satoshi Makihata、Katsunobu Kawahara 等人：“异气管移植的实验研究”呼吸外科演示。

三上公治、川原克信他5名: "凍結ブタ-イヌ気管移植の実験的検討" 日本胸部外科学会誌. 45(増). 1407- (1997)

Koji Mikami、Katsunobu Kawahara 等 5 人：“冷冻猪-狗气管移植的实验研究”日本胸外科学会杂志 45（en）。

Inutsuka K,Kawahara K,et al: "Reconstruction of trachea and carina with immediate or cryopreserved allografts in dogs" Annals of Thoracic Surgery. 62. 1480-1484 (1996)

Inutsuka K、Kawahara K 等人：“用立即或冷冻保存的狗同种异体移植物重建气管和隆突”胸外科年鉴。