Optical mapping/imaging of neural activity in the spinal cord : investigation for possible mechanisms of dysesthesia

脊髓神经活动的光学测绘/成像：调查感觉迟钝的可能机制

基本信息

批准号：
11671422
负责人：
MOMOSE-SATO Yoko
金额：
$ 2.5万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

To examine the possible mechanisms for dysesthesia, we investigated spatiotemporal patterns of neuronal pathway/network activity in embryonic and adult spinal cords, using multiple-site optical recording/imaging techniques.In experiments using embryos, spinal cords were dissected from chick and rat embryos, and transverse slice preparations were prepared with the 13th cervical spinal nerve or with the 2nd or 5th lumbosacral spinal nerve intact. The slice preparations were stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). Transmembrane voltage-related optical (dye-absorbance) changes evoked by spinal nerve stimulation with positive square current pulses using a suction electrode were recorded simultaneously from many loci in the preparation, using a 128- or 1020-element photodiode array. Optical responses were detected from dorsal and ventral regions corresponding to the posterior (dorsal) and anterior (ventral) gray matters. The optical signals were composed of two c … More omponents, fast spike-like and slow signals. In the dorsal region, the fast spike-like signal was identified as the presynaptic action potential in the sensory nerve, and the slow signal as the postsynaptic potential. In the ventral region, the fast spike-like signal reflected the antidromic action potential in motoneurons, and the slow signal was related to the postsynaptic potential evoked in the motoneuron.In experiments using adult spinal cords, we made an examination of the feasibility of an intrinsic optical imaging evoked by spinal nerve stimulation in the in vivo rat spinal cord. Adult rats were anesthetized by Urethane and immobilized by d-tubocurarine. Laminectomy was performed between C5 and Th1 to expose the cervical spinal cord. Median and ulnar nerves were dissected and bipolar electrodes were implanted in the forelimb. Changes in optical reflectance were recorded from spinal cord in response to median and ulnar nerve stimulation using a differential video acquisition system. In the dorsal region of the cervical spinal cord, the intrinsic optical signals were detected clearly between C6 and C8, and the amplitude of the signals increased in proportion to the intensity of stimulation. The response evoked by peripheral nerve stimulation in the dorsal cervical spinal cord is suggested to be neural activities of the primary afferent and the postsynaptic neurons in the dorsal horn. Less

To examine the possible mechanisms for dysesthesia, we investigated spatial temporal patterns of neuronal pathway/network activity in embryonic and adult spinal cords, using multiple-site optical recording/imaging techniques.In experiments using embryos, spinal cords were dissected from chick and rat embryos, and transverse slice preparations were prepared with the 13th cervical spinal nerve or with the 2nd or 5th lumbosacral脊神经完整。将切片制剂用电压敏感的梅罗氨基 - 羟氨烷染料（NK2761）染色。使用128或1020-元素的光电二极管阵列记录了跨膜电压相关的光学（染料吸收）的变化，该变化是通过脊柱神经刺激和使用吸气电极正方向脉冲使用吸气电极的正方形脉冲进行的。从背侧和腹侧区域检测到光学反应，对应于后（背侧）和前（腹侧）灰质。光学信号由两个C…更多的组件组成，类似尖峰和缓慢的信号。在背区域，快速尖峰样信号被确定为感觉神经中突触前动作电位，而缓慢的信号则是突触后电位。在腹侧区域，快速的尖峰样信号反映了运动神经元中的抗体作用潜力，缓慢的信号与运动神经元中引起的突触后潜力有关。在使用成人脊髓的实验中，我们对脊柱固有成像的可行性检查了脊柱固定成像在spinal nerve nerve inve inve in vivo corn in vivo ratin中的可行性。成年大鼠用氨基甲异烷麻醉，并被D-纤维嘌呤固定。在C5和TH1之间进行椎板切除术，以暴露宫颈脊髓。解剖中位和尺神经，并将双极电极植入前肢。使用差异视频采集系统，通过脊髓和尺神经刺激的中位数记录了光学反射率的变化。在颈脊髓的背侧区域，在C6和C8之间清楚地检测到了固有的光学信号，并且信号的放大器与刺激强度成比例增加。表背脊髓中周围神经刺激引起的反应被认为是背角中主要传入的神经活性和突触后神经元的神经活性。较少的