Direct Gene Transfer Into Articular Cartilage By Gene Gun

通过基因枪将基因直接转移到关节软骨中

• 批准号: 11671438
• 负责人: UMEHARA Takashi
• 金额: $ 0.83万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671438/
• 关键词: gene; cartilage; gene gun; 軟骨細胞; サイトカイン

We investigated whether marker gene can be transferred into the articular cartilage with a gene gun.Articular cartilage was harvested from the femoral head and femoral condyle at the total hip replacement and total knee replacement. Cartilage tissue was dissected into slices, measuring 10×10 mm. A bacterial marker gene, lac Z, which expresses the enzyme β-galactasidase, was coated on gold particle. The articular cartilage was bombared with a Helios Gene Gun (100 psi, 200 psi, 300 psi, 400 psi). The carilage slice was cultured as an organ culture. b-galactasidase activity was evaluated 24 to 48 hours after bombardment.b-galactasidase activity after 24 hours was slightly higher than that after 48 hours, however, those activities were not significantly higher than control. Pressure at the bombardment did not influence the β-galactasidase activity. Histological examination revealed that the gold particles with lac Z gene did not penetrate the articular cartilage.The present study demonstated that the particle bombardment using a Helios Gene Gun cannot transfer the marker gene into the articular cartilage because it cannot make the gold particle penetrate the articular cartilage tissue.

本研究采用基因枪技术将标记基因导入关节软骨组织中，并在全髋关节置换术和全膝关节置换术中取股骨头和股骨髁软骨组织。将软骨组织切成10×10 mm的切片，将表达β-半乳糖苷酶的细菌标记基因lac Z包被在金颗粒上。用Helios基因枪（100 psi、200 psi、300 psi、400 psi）轰击关节软骨。将carilage切片培养为器官培养物。轰击后24 ~ 48小时测定β-半乳糖苷酶活性，轰击后24小时的β-半乳糖苷酶活性略高于轰击后48小时的β-半乳糖苷酶活性，但与对照相比，这些活性并不显著。轰击压力不影响β-半乳糖苷酶活性。组织学检查显示，携带lac Z基因的金粒子不能穿透关节软骨，本研究表明，使用Helios基因枪的粒子轰击不能使标记基因进入关节软骨，因为它不能使金粒子穿透关节软骨组织。

项目成果

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