Direct Gene Transfer Into Articular Cartilage By Gene Gun

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We investigated whether marker gene can be transferred into the articular cartilage with a gene gun.Articular cartilage was harvested from the femoral head and femoral condyle at the total hip replacement and total knee replacement. Cartilage tissue was dissected into slices, measuring 10×10 mm. A bacterial marker gene, lac Z, which expresses the enzyme β-galactasidase, was coated on gold particle. The articular cartilage was bombared with a Helios Gene Gun (100 psi, 200 psi, 300 psi, 400 psi). The carilage slice was cultured as an organ culture. b-galactasidase activity was evaluated 24 to 48 hours after bombardment.b-galactasidase activity after 24 hours was slightly higher than that after 48 hours, however, those activities were not significantly higher than control. Pressure at the bombardment did not influence the β-galactasidase activity. Histological examination revealed that the gold particles with lac Z gene did not penetrate the articular cartilage.The present study demonstated that the particle bombardment using a Helios Gene Gun cannot transfer the marker gene into the articular cartilage because it cannot make the gold particle penetrate the articular cartilage tissue.