Anti-tumor Effect of Plasminogen Activator Inhibitor Derived from Shark Cartilage

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Anti-tumor substances were extracted from shark cartilage with 1M GuHCl and partially purified by ultrafiltration, gel-chromatography, affinity-chromatograry and SDS-PAGE. This extracted fraction was called as "Cartilage-derived plasminogen activator inhibitor: CD-PAI". The Physiological activity of the CD-PAI to endothelial cell and tumor cells were assayed by in vitro and in vivo study. In in vitro studies, the CD-PAI had inhibitory activities against endothelial cell proliferation, migration and tube formation. And also, the CD-PAI showed inhibitory activity against tumor cell invasion. On the other hand, tumor cell proliferation was not inhibited by the CD-PAI. In in vivo studies, the CD-PAI inhibited tumor growth implanted in mice. Theses results suggest that the tumor inhibitory mechanisms of the CD-PAI are anti-angiogenesis and anti-tumor invasion. We felt that further studies concerning with analysis of protein sequence and distribution of the CD-PAI in human normal tissue.