THE INFLUENCE OF INHALATIONAL ANESTHETICS ON THE ENERGY METABOLISM AND CREATIN KINASE SYSTEM OF BRAIN DURING BRAIN ISCHEMIA

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We previously reported that halothane (Hal) delayed the recoveries in cerebral intracellular pH (pHi) and [ATP] in comparison with isoflurane (Iso) and sevoflurane (Sev) anesthesia after cerebral ischemia in the rat model. We did not find any differences in the recoveries in [inorganic P] nor [phosphocreatine (PCr)], therefore we hypothesized that Hal affected the creatine kinase (CK) forward reaction (PCr+ADP→Cr+ATP).METHODS : With using magnetic resonance spectroscopy, we further investigated whether Hal affected the brain energy metabolism during and after cerebral ischemia dose-dependently. Wistar rats were divided into 3 groups : 1% Hal-, 2% Hal-, or 1.5% Iso-treated (n=7). After 9 min of cerebral ischemia by bilateral carotid ligation and blood withdrawal, we continued the observation up to 2 hrs. We then measured the pseudo first-order rate constant (kf) of CK forward reaction in Hal/Iso anesthesia determined by MR saturation transfer technique under normoxic condition.RESULTS : Wistar rats were divided into 2 groups Hal delayed the recoveries in pHi and [ATP] in a comparison with Iso group but in the two Hal groups, we could not find the dose-dependent effect between 2 Hal groups. There were no differences between the both anesthetic conditions but significant difference in the values of kf (0.24±0.05 in Hal and 0.30±0.04 in Iso anesthesia).CONCLUSION : Halothane might impair the brain energy balance and we concluded that halothane has an undesirable property for neuroanesthesia with the risk of cerebral ischemia.