Role of superoxide dismutase in the prolongation of the life span of the corpus luteum

超氧化物歧化酶在延长黄体寿命中的作用

• 批准号: 11671623
• 负责人: SUGINO Norihiro
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671623/
• 关键词: corpus luteum; SOD; superoxide radical; apoptosis; life span; progesterone; testosterone; placental luteotropin; luteotropin

1. The first study was undertaken to investigate whether suppression of intracellular Cu, Zn-SOD activity influences luteal function. To suppress SOD activity, dispersed rat luteal cells were incubated with Cu, Zn-SOD antisense oligonucleotides. The 48-h treatment with antisense oligonucleotides (10 μM) inhibited both SOD activity by 50% and progesterone concentrations in the medium. This inhibitory effect was completely blocked by the simultaneous addition of an antioxidant. Treatment with antisense oligonucleotides caused no significant change in the percentage of apoptotic cells. It is concluded that the decrease in intracellular SOD activity inhibits progesterone production by rat luteal cells, which may be mediated by superoxide radicals, suggesting that intracellular SOD plays important roles in the regulation of luteal function.2. (1) The second study was undertaken to investigate whether SOD is related to pregnancy-induced maintenance of luteal function. Placentae obtained from … More rats on day 12 of pregnancy were incubated for 24h, and the supernatant was used as placental luteotropins. Pseudopregnant rats were given the placental incubation medium from day 9 to day 12 of pseudopregnancy. Serum progesterone concentrations and luteal SOD mRNA and activity were increased by this treatment on day 12. Testosterone in the placental incubation medium acted on the corpus luteum without conversion to estrogen. (2) The third study was undertaken regarding the involvement of SOD in the human luteal function. SOD expression in the corpus luteum of early pregnancy was significantly higher than that in the mid-luteal phase corpus luteum. The incubation of the mid-luteal phase corpus luteum with hCG increased both luteal SOD expression and progesterone production. Collectively, it is concluded that placental luteotropins increased SOD expression in the corpus luteum and stimulated progesterone production, suggesting that SOD is involved in the maintenance of the luteal function by placental luteotropins. Less

1. 第一项研究旨在探讨抑制细胞内 Cu、Zn-SOD 活性是否会影响黄体功能。为了抑制 SOD 活性，将分散的大鼠黄体细胞与 Cu、Zn-SOD 反义寡核苷酸一起孵育。用反义寡核苷酸 (10 μM) 处理 48 小时，可抑制 50% 的 SOD 活性和培养基中的孕酮浓度。同时添加抗氧化剂可以完全阻断这种抑制作用。用反义寡核苷酸处理没有引起凋亡细胞百分比的显着变化。结论：细胞内SOD活性降低抑制大鼠黄体细胞产生孕酮，其作用可能是由超氧自由基介导的，提示细胞内SOD在黄体功能调节中发挥着重要作用。 2． (1) 第二项研究旨在调查 SOD 是否与妊娠诱导的黄体功能维持有关。取妊娠第12天大鼠胎盘，培养24h，取上清液作为胎盘促黄体素。假孕大鼠在假孕第9天至第12天给予胎盘培养液。第12天，通过这种处理，血清孕酮浓度和黄体SOD mRNA和活性增加。胎盘培养介质中的睾酮作用于黄体，但不转化为雌激素。 (2) 第三项研究是关于SOD 与人类黄体功能的关系。妊娠早期黄体中SOD表达量显着高于黄体中期黄体。黄体中期黄体与 hCG 的孵育增加了黄体 SOD 表达和黄体酮产生。总的来说，胎盘促黄体素可增加黄体中 SOD 的表达并刺激黄体酮的产生，表明 SOD 参与胎盘促黄体素对黄体功能的维持。较少的

项目成果

Takiguchi S.: "Rescue of the corpus luteum and an increase in luteal superoxide dismutase expression induced by placental luteotropins in the rat : action of cestosteronre without conversion to estrogen"Biology of Reproduction. 62(in press). (2000)

Takiguchi S.：“大鼠黄体的拯救和胎盘促黄体素诱导的黄体超氧化物歧化酶表达的增加：睾甾酮的作用而不转化为雌激素”生殖生物学。

Sugino N.: "Expression of Bcl-2 and Bax in the human corpus luteum during the menstrual cycle and in early pregnancy regulation by human chorionic gonadotropin."Journal of Clinical Endocrinology and Metabolism. 85. 4379-4386 (2000)

Sugino N.：“月经周期和妊娠早期受人绒毛膜促性腺激素调节的人黄体中 Bcl-2 和 Bax 的表达。”临床内分泌与代谢杂志。

Sugino N.: "Expression of vascular endothelial growth factor and its receptors in the human corpus luteum during the menstrual cycle and in early pregnancy."Journal of Clinical Endocrinology and Metabolism. 85. 3919-3924 (2000)

Sugino N.：“月经周期和妊娠早期血管内皮生长因子及其受体在人黄体中的表达。”临床内分泌与代谢杂志。

Sugino N.: "Superoxide dismutase expression in the human capus luteum during the menstrual cycle and in early pregnancy"Molecular Human Reproduction. 6(in press). (2000)

Sugino N.：“月经周期和妊娠早期人类黄体中超氧化物歧化酶的表达”《分子人类生殖》。

Sugino N: "Decreased superoxide dismutase. expression and increased concerntrations of lipid peroxide and prostaglandin F12α in the decidum of failed pregnanoy."Molecular Human Reproduction. 6. 642-647 (2000)

Sugino N：“妊娠失败的蜕膜中超氧化物歧化酶的表达降低，脂质过氧化物和前列腺素 F12α 的浓度增加。”《分子人类生殖》6. 642-647 (2000)。