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Changes in blood vessel stability and its regulation in the corpus luteum during pregnancy in rats

大鼠妊娠期黄体血管稳定性变化及其调控

基本信息

批准号：
15591753
负责人：
SUGINO Norihiro
金额：
$ 2.24万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591753/
关键词：
corpus luteum angiogenesis vascular leakage angiopoietin Tie-2 regenerative medicine estradiol estradiol Angiopoietin

项目摘要

The present study investigates changes in blood vessel stability and its regulation in the corpus luteum (CL) during pregnancy in rats. First, blood vessel stability in the CL was evaluated on days 3, 7. 9, 12, 15 and 21 of pregnancy based on the vascular leakage, which was quantified by Evans blue assay. Vascular leakage was highest on day 3, thereafter decreased until day 15 and increased on day 21 again, suggesting that blood vessels in the CL are not stabilized on day 3 yet, stabilized during mid-pregnancy, and destabilized on day 21 of pregnancy. Secondly, to study the regulation of blood vessel stability, expression of angiopoietins was examined in the CL during pregnancy. Immunohistochemical study showed both angiopoietin-1 (Ang-1) and Ang-2 expressions in luteal cells. Ang-1 mRNA and protein levels were significantly higher on days 12 and 15 than those on days 3 and 21, whereas there was no significant change in Ang-2 expression. Since estradiol stimulates angiogenesis and contributes to CL development during mid-pregnancy, we finally studied whether estradiol regulates blood vessel stability and angiopoietin expression. Rats undergoing hypophysectomy-hysterectomy (Hypox-Hect) on day 12 were treated with estradiol until day 15. Vascular leakage was increased and Ang-1 expression was decreased by Hypox-Hect, and these effects were completely reversed by estradiol treatment. In conclusion, blood vessel stability in the CL is likely to be associated with CL development and CL regression, and may be regulated by angiopoietins. Estradiol contributes to blood vessel stabilization via Ang-1 in the CL during mid-pregnancy.

本研究探讨了妊娠期大鼠黄体血管稳定性的变化及其调控。首先，在第3天和第7天对CL的血管稳定性进行评估。根据妊娠第9、12、15、21期血管渗漏情况，采用Evans蓝法定量。血管渗漏在第3天最高，此后减少至第15天，第21天再次增加，说明CL血管在第3天尚未稳定，在妊娠中期稳定，在妊娠第21天不稳定。其次，为了研究妊娠期CL对血管稳定性的调节作用，我们检测了血管生成素的表达。免疫组化研究显示黄体细胞中血管生成素-1 （ang1）和ang2均有表达。与第3、21天相比，第12、15天Ang-1 mRNA和蛋白水平显著升高，而Ang-2表达无显著变化。由于雌二醇刺激血管生成并促进妊娠中期CL的发展，我们最终研究了雌二醇是否调节血管稳定性和血管生成素的表达。在第12天进行垂体切除-子宫切除术（Hypox-Hect）的大鼠给予雌二醇治疗至第15天。Hypox-Hect可增加血管渗漏，降低Ang-1表达，雌二醇治疗可完全逆转这些影响。综上所述，CL的血管稳定性可能与CL的发展和消退有关，并可能受血管生成素的调节。雌二醇通过妊娠中期CL中的Ang-1促进血管稳定。

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Reactive oxygen radicals in ovarian physiology.

卵巢生理学中的活性氧自由基。

DOI：
发表时间：
2005
期刊：
Reproductive Medicine and Biology 4
影响因子：
0
作者：
Sugino N.
通讯作者：
Sugino N.

総説;活性酸素を介した生殖戦略:子宮と卵巣における活性酸素とその消去系酵素の役割

回顾；活性氧介导的生殖策略：活性氧及其清除酶在子宫和卵巢中的作用

DOI：
发表时间：
2004
期刊：
山口医学 53
影响因子：
0
作者：
Sugino N.;工藤美樹;Suganuma T et al.;工藤美樹;Sugino N et al.;Kajiyama H et al.;工藤美樹;Takiguchi S et al.;Terauchi M et al.;杉野法広
通讯作者：
杉野法広

Withdrawal of ovarian steroids stimulates prostaglandin F2a production through nuclear factor-kB activation via oxygen radicals in human endometrial stromal cells.

取消卵巢类固醇会通过人子宫内膜基质细胞中的氧自由基激活核因子-kB，刺激前列腺素 F2a 的产生。