Effect of neuropeptides on postmenopausal bone metabolism

尽管人们对骨质疏松症越来越感兴趣，一些有效的药物也被引入临床领域，但其病理生理机制仍不清楚。已知有许多因素导致骨质疏松症，如雌激素缺乏，许多其他因素也有助于对雌激素的反应。在本研究中，我们关注神经肽受体，并分析了这些变化与激素环境，特别是雌激素的小鼠模型。用RT-PCR方法分析了12种神经肽受体m-RNA在Sham、OVX和E2替代ddy小鼠骨骼中的表达。检测VIP-2、CGRP-RCP和ALP受体在骨组织中的表达。OVX组VIP-2的相对表达约为Sham组的1.75倍。有显著差异。雌二醇组与假手术组之间无明显差异。OVX组CGRP-RCP的相对表达低于E2替代组(0.55倍)。骨组织中部分神经肽受体的表达随雌激素水平的变化而变化，提示神经肽可能在绝经后骨代谢中起重要作用。

In spite of increasing interest in osteoporosis and several effective drugs introduced to clinical fields, its pathophysiology is not clarified yet. Many factors are known to contribute to osteoporosis, such as estrogen deficiency, and many other factors also contribute to the response to estrogen. In this study, we paid attention to neuropeptide receptors and analyzed those changes with hormonal environment, especially estrogen with mouse models.Expressions of 12 neuropeptide receptors m-RNA in bone were analyzed on Sham, OVX and E2 replaced ddY mouse with RT-PCR method. The expressions of VIP-2, CGRP-RCP, and ALP receptors were detected on bone. The relative expression of VIP-2 in OVX group was about 1.75 folds higher than that in Sham group. There was a significantly difference. No difference was found between E2 replaced and Sham groups. The relative expression of CGRP-RCP in OVX group was lower (0.55 folds) than that in E2 replaced group. On the other hands, the expression of ALP did not differ among three groups.Some neuropeptide receptors on bone changed with respect to estrogen status and these results suggested that neuropeptides may play an important role in postmenopausal bone metabolism.