Changes in the expression of adenylyl cyclase and G-protein mRNA and the regulation system in myometrium

1妊娠及产后大鼠肌层腺苷酸环化酶（adenylyl cyclase， AC）和g蛋白mrna的表达●ACs （AC2、AC4、AC6、AC7、AC9）表达水平在妊娠期间升高，在妊娠期及分娩后降低。在妊娠第17天，每种类型AC的表达水平均达到最高。●Gs蛋白表达量仅在妊娠第17天升高，其他日期无变化妊娠和产后大鼠肌层AC活性与上述研究结果一致，在妊娠过程中，基础肌层AC活性升高，而在妊娠期和产后肌层AC活性降低●雌激素(E)、雌激素(E)、黄体酮(P)、黄体酮(P)分别使AC2、AC4、AC9 mrna表达升高。●AC6、AC7 mrna的表达不受E、p的影响，g mrna的表达随p的增加而增加。④妊娠大鼠模型中仅一侧子宫AC mrna的表达：妊娠侧与非妊娠侧相比，AC2 mrna表达增加。●妊娠侧和非妊娠侧小鼠AC4、AC6、AC7、AC9 mrna表达无明显变化。这些结果表明，1。ACs和Gs mrna的表达受不同性激素的影响。除雌激素和黄体酮外，AC mrna的表达可能受多种因素的影响。ac2mrna的表达可通过多种激素的局部分泌或拉伸刺激诱导。

1 Expression of adenylyl cyclase (AC) and G-protein mRNAs in rat myometrium during the course of pregnancy and after delivery● The level of expression of ACs (AC2, AC4, AC6, AC7 and AC9) increased during the course of pregnancy and diminished near term and after delivery. The highest level of expression in each type of AC was consistently seen on day 17 of pregnancy.● The level of expression of Gs protein increased only on day 17 of pregnancy, and it did not change on the other dates.2 AC activities in rat myometrium during the course of pregnancy and after delivery● In agreement with above findings, both basal and forscolin-stimulated AC activities increased during the course of pregnancy and diminished near term and after delivery.3 Changes in the expression of AC and G-protein mRNAs in rat castrated and hormone administrated model● The expression of AC2, AC4 and AC9 mRNAs increased by estrogen (E), E and progesterone (P) and P, respectively.● The expression of AC6 and AC7 mRNAs did not change by E or P.● The expression of Gs mRNAs increased by P.4 Expression of AC mRNAs in rat model that is pregnant only in the one side of the uterus● The expression of AC2 mRNAs increased in the pregnant side coropared to that in the non-pregnant side.● The expression of AC4, AC6, AC7 and AC9 mRNAs did not change in the both pregnant and non-pregnant sides.These results suggest that1. The expression of ACs and Gs mRNAs is influenced by the different sex hormones.2. There is a possibility that the expression of AC mRNAs is influenced by several factors except to estrogen and progesterone.3. The expression of AC2 mRNA seeros to induct by the local secretion of several hormones or the stretched stimulation.