The Analysis of glicosyltransferase involved in the cell biological function of gynecological cancers

Lewis^b （Le^b）抗原（典型的I型碳水化合物链）的表达水平与人类子宫内膜癌患者的5年生存率有关。我们还发现糖基转移酶控制着糖链的表达。在本研究中，我们研究了糖基转移酶在妇科肿瘤细胞生物学功能中的作用。研究了β1-4半乳糖转移酶（β1-4GT）基因产物在11株妇科癌细胞中的表达。Northern blot和western blot检测A4、7Kb mRNA和蛋白表达。在子宫癌和卵巢癌细胞中检测到强烈的β1-4GT mRNA信号，而子宫内膜癌细胞中β1-4GT mRNA水平极低。这些结果表明，β1-4 mRNA和β1-4基因产物在宫颈癌和卵巢癌细胞中的表达高于子宫内膜癌细胞。然后将人β1-4GT cDNA反义转染到子宫内膜和卵巢癌细胞系。我们进一步分析了β1-4GT在子宫内膜和卵巢癌细胞中的低表达的生物学效应。体外研究表明，与载体对照细胞相比，细胞生长明显减少，对层粘连蛋白和IV型胶原的粘附活性降低。这些数据表明，β1-4GT基因产物的调控在很大程度上影响了妇科癌细胞的生物学特性。

The expression level of Lewis^b (Le^b) antigen, typical type I carbohydrate chain, was previously shown to correlate with the five-year survival rate of human endometrial cancer patients. We also have shown that the expression of carbohydrate chain is controlled by glycosyltransferase. In this study, we investigated the Analysis of glycosyltransferase involved in the cell biological function of gynecological cancers. Expressions of β1-4 galactosyltransferase (β1-4GT) gene products in eleven gynecological cancer cell line was examined. A4, 7Kb mRNA and protein were detected by Northern blot and western blot analysis. An intense β1-4GT mRNA signal was detected in uterine cervical and ovarian cancer cells, whereas the level of β1-4GT mRNA was very low in uterine endometrial cancer cells. These results suggested that the expression of β1-4 mRNA and β1-4 gene products are higher in cervical and ovarian cancer cells than in endometrial cancer cells. Then we transfectecd human β1-4GT cDNA antisense into uterine endomertial and ovarian cancer cell lines. Further we analyzed the biological effects of underexpression of β1-4GT in endometrial and ovarian cancer cells. In vitro study has showed a significant decrease of cell growth and decrease of adhesion activity to laminin and collagen type IV compared to vector control cells.These data indicate that the regulation of β1-4GT gene products many charged the biological properties of gynecological cancer cells.