Neuroanatomical study on the participation of carbon monoxide in the laryngeal innervation

一氧化碳参与喉神经支配的神经解剖学研究

基本信息

批准号：
11671697
负责人：
HISA Yasuo
金额：
$ 2.3万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671697/
关键词：
larynx nervous system carbon monoxide HO-2 神経伝達物質 heme oxygenase 神経解剖免疫組織化学 Carbon monoxide beme oxygenase

项目摘要

1.Colocalization of NADPHd with VIP or HO-2 in the intramuscular neurons of the intrinsic laryngeal musclesDouble staining utilizing NADPHd histochemistry and immunohistochemistry for VIP or HO-2 was performed in the canine cricothyroid and posterior cricoarytenoid muscles. Some of the NADPHd positive neurons in the intrinsic laryngeal muscles were VIP positive, and NADPHd negative neurons were also seen among the VIP positive neurons. None of the NADPHd positive neurons were HO-2 positive. The intramuscular neurons may consist of subgroups of cells with different functions, since colocalization of VIP was not seen in all the NADPHd positive neurons. Although NO, CO, and VIP are known to exist in parasympathetic neurons, the intramuscular neurons seen in this study differ from the parasympathetic intralaryngeal neurons, since many of the later colocalize CO and NO.2.Neurochemical substances in neurons of the intralaryngeal gamglia Localization of neuropeptides, HO-2 and nNOS was studied in the rat and canine intralaryngeal neurons using immunohistochemistry and NADPHd histochemistry. Colocalization of HO-2 and NADPHd was seen in some of the canine intralaryngeal neurons. The known ratio of HO-2 positive neurons among the NADPHd positive neurons in the esophagus is 70-80%, and the ratio of NADPHd positive neurons among the HO-2 positive neurons is 35% in the cervical and thoracic esophagus and 50% in the abdominal esophagus. Therefore the intralaryngeal neurons do not differ greatly in content of gaseous neurotransmitters from parasympathetic esophageal intramural neurons.

1.用NADPHD组织化学和免疫组织化学的内在性喉部肌肉肌肉染色的NADPHD用VIP或HO-2的NADPHD在肌肉或HO-2的固有性喉部神经元中进行了钙化化。内在喉肌中的某些NADPHD阳性神经元为VIP阳性，并且在VIP阳性神经元中也可以看到NADPHD阴性神经元。 NADPHD阳性神经元都没有HO-2阳性。肌内神经元可能由具有不同功能的细胞亚组组成，因为在所有NADPHD阳性神经元中均未看到VIP的共定位。尽管已知NO，CO和VIP存在于副交感神经元中，但这项研究中看到的肌内神经元与副交感神经内神经元不同，因为许多后来的共定位CO和No.2.Neurochemical co.2.neurochemical co.2.neurochemicalco.。使用免疫组织化学和NADPHD组织化学的咽内神经元。在一些犬内神经元中可以看到HO-2和NADPHD的共定位。食管中NADPHD阳性神经元中HO-2阳性神经元的已知比率为70-80％，HO-2阳性神经元中NADPHD阳性神经元的比率为35％，在宫颈食管和胸腔食管中为50％，在腹部食管中为50％。因此，在副交感神经食管内神经元的气态神经递质含量中，脑内神经元没有很大差异。