FUNCTION OF AQUAPORIN OF RETINAL MUELLER CELLS IN RETINAL EDEMA AND DETACH MENT

视网膜Mueller细胞水通道蛋白在视网膜水肿和脱离中的作用

• 批准号: 11671745
• 负责人: KITAOKA Takashi
• 金额: $ 2.18万
• 依托单位: DEPARTMENT OF OPHTHALMOLOGY, NAGASAKI UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671745/
• 关键词: Aquaporin; Mueller cells; macular edema; retinal detachment; retinal edema; immunohistochemistry; macular hole; internal limiting membrane; 網膜ミュラー細胞; レーザー光凝固; 糖尿病網膜症

Aquaporins (AQPs) are water channel proteins and distribute widely in the eye. AQPs are reported to exist in Mueller cells and may play a role in the absorption of subretinal fluid and/or retinal edema.We demonstrated that AQP 4 exists in the retinal Mueller cells by using light microscopic immunohistochemistry. Furthermore, AQP 4 was revealed to exist in the plasma membrane of Mueller cells by immunoelectron microscopy.It is difficult to make the experimental retinal edema or retinal detachment in the rat eye. In this study, we performed retinal laser photocoagulation and edema occurred around the laser burns. In the retinal edema after laser photocoagulation, the expression of AQP 4 was increased around the retinal edema by immunohistochemistry.Internal limiting membrane (ILM) peeling was widely used in recent surgery of retinal macular hole or macular edema. ILM peeling facilitated the closure of macular hole and absorption of macular edema, but sometimes cystoid macular edema occurred for long time after surgery. We performed the ILM peeling and the immunohistochemistry for AQP 4 in surgically removed ILM.Colloidal golds which corresponded to AQP 4 location existed in the inner surface of surgically removed ILM.These results suggest AQP 4 in Mueller cells may play a role in absorption of retinal edema or subretinal fluid, and the damage of Mueller cells may cause the cystoid macular edema.

水通道蛋白(AQPs)是一种水通道蛋白，广泛分布于眼部。AQP存在于视网膜Mueller细胞中，可能在视网膜下积液和/或视网膜水肿的吸收中起作用。免疫电子显微镜显示AQP-4存在于大鼠视网膜Mueller细胞质膜中，不易造成大鼠实验性视网膜水肿或视网膜脱离。在本研究中，我们进行了视网膜激光光凝以及激光烧伤周围发生的水肿症。在激光光凝后的视网膜水肿区，免疫组织化学显示AQP-4在视网膜水肿区周围表达增加，内界膜剥离术在近期黄斑裂孔或黄斑水肿术中得到广泛应用。ILM剥离有利于黄斑裂孔的闭合和黄斑水肿的吸收，但有时黄斑囊样水肿会在术后较长时间出现。我们对手术切除的ILM进行了ILM剥离和AQP-4免疫组织化学染色，发现在手术切除的ILM内表面有与AQP-4定位相对应的胶体金，提示Mueller细胞中的AQP-4可能在吸收视网膜水肿或视网膜下液中起作用，Mueller细胞的损伤可能导致黄斑囊样水肿。

项目成果

Kitaoka T,Fujimoto K,Misaka T,Miyamura N,Amemiya T: "Cytochemical demonstration of Aquaporin 4 in retinal edema."Invest Ophthalmol Vis Sci. 40(S). 583 (1999)

Kitaoka T、Fujimoto K、Misaka T、Miyamura N、Amemiya T：“水通道蛋白 4 在视网膜水肿中的细胞化学演示。”Invest Ophasemol Vis Sci。

Kitaoka T: "Internal limiting membrane peeling"J Eye. 16. 1355-1360 (1999)

北冈T：“内界膜剥离”J Eye。

Kitaoka T, Oba K, Amemiya T: "Comparison of three methods of internal limiting membrane peeling for idiopathic macular hole."Jpn J Clin Ophthalmol. 54. 883-886 (2000)

Kitaoka T、Oba K、Amemiya T：“特发性黄斑裂孔内界膜剥离的三种方法的比较。”Jpn J Clin Ophthalmol。

Mera A, Kitaoka T, Taniguchi H, Amemiya T: "Hyperbaric oxygenation therapy improves visual field defect after macular hole surgery."Invest Ophthalmol Vis Sci. 40(S). 699 (1999)

Mera A、Kitaoka T、Taniguchi H、Amemiya T：“高压氧治疗可改善黄斑裂孔手术后的视野缺损。”Invest Ophasemol Vis Sci。

Kitaoka T,Fujimoto K,Misaka T,Miyamura N,Amemiya T: "Cytochemical demonstration of Aquaporin 4 in retina edema."Invest Ophthalmol Vis Sci. 40(4). S583 (1998)

Kitaoka T、Fujimoto K、Misaka T、Miyamura N、Amemiya T：“水通道蛋白 4 在视网膜水肿中的细胞化学演示。”Invest Ophasemol Vis Sci。