THERAPY FOR MACULAR EDEMA AND RETINAL DETACHMENT BY USING WATER CHANNEL PROTEIN AQUAPORIN.

使用水通道蛋白水通道蛋白治疗黄斑水肿和视网膜脱离。

• 批准号: 14571678
• 负责人: KITAOKA Takashi
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571678/
• 关键词: Aquaporin; retina; macula; internal limiting membrane; diabetes mellitus; vitrectomy; analysis; electron microscopy; 黄斑部; 内境界膜; 黄斑円孔; 黄斑浮腫; 免疫組織化; ミュラー細胞

Aquaporin (AQP) is a water channel protein which has been preserved through animals, plants, and microorganelles. In ophthalmological field, the eye and AQP have important relationship,such as that AQP exists in ciliary body, and that AQP 4 and 5 are related to the production and secretion of tear film. It was also reported that AQP 4 exists in the plasma membrane of Mueller cells. We reported that AQP exists in the internal limiting membrane which excised during vitrectomy. We discovered that AQP is more expressed in the internal limiting membrane of diabetic patient than that of non-diabetic patient. This may indicate that AQP is increasing to absorb edema or that AOP increase edema.On the other hand, it is well known that visual field defect occur after vitrectomy and we reported that hyperbaric oxygenation therapy decreased this visual field defect. This may indicate the expression of AQP is affected by oxygen. After that, we reported that the wall between vitreous pocket and Cloquet's canal may cause several diseases such as vitreo-retinal traction syndrome and retinal edema by using Triamcinolone acetonide. Finally, we used ion microscope to detect the ion and organic which showed the possibility to observe the distribution of AQP.

水通道蛋白(Aquaporin，AQP)是一种通过动物、植物和微细胞器保存的水通道蛋白。在眼科领域，眼与水通道蛋白有重要的关系，如水通道蛋白存在于睫状体，水通道蛋白4和5与泪膜的产生和分泌有关。也有报道水通道蛋白4存在于Mueller细胞的质膜上。我们报道了AQP存在于玻璃体切除过程中切除的内界膜中。我们发现AQP在糖尿病患者的内界膜中的表达高于非糖尿病患者。这可能表明AQP正在增加以吸收水肿或AOP增加了水肿。另一方面，众所周知，玻璃体切除后会发生视野缺陷，我们报道了高压氧治疗减少了这种视野缺陷。这可能表明AQP的表达受氧的影响。在此之后，我们报道了使用曲安奈德后，玻璃体袋和克洛奎管之间的壁可能会导致多种疾病，如玻璃体视网膜牵引综合征和视网膜水肿。最后，我们用离子显微镜对水中的离子和有机物进行了检测，显示了观察水中水通道蛋白分布的可能性。

项目成果

Kitaoka T, Taniguchi H, Miyamura N, Amemiya T.: "Aquaporin expression of internal limiting membrane in diabetic macular edema."Invest Ophthalmol Vis Sci. 44. E-abstract372 (2003)

Kitaoka T、Taniguchi H、Miyamura N、Amemiya T.：“糖尿病性黄斑水肿中内界膜的水通道蛋白表达。”Invest Ophasemol Vis Sci。

Zong-Yang Lu, Kishikawa Y,.Tsuda Y., Kitaoka T, Amemiya T.: "Macular internal limiting membrane : ultrastructure and trace elements"Rinsho Ganka (Jpn J Clin Ophthalmol). 57(3). 281-284 (2003)

卢宗阳，岸川Y，津田Y.，北冈T，Amemiya T.：“黄斑内界膜：超微结构和微量元素”Rinsho Ganka（Jpn J Clin Ophthalmol）。

Kuroki AM, Kitaoka T.Taniguchi H, Amemiya T.: "Hyperbaric oxygen therapy reduces visual field defect after macular hole surgery."Ophthalmic Surg Lasers. 33. 200-206 (2002)

Kuroki AM、Kitaoka T.Taniguchi H、Amemiya T.：“高压氧疗法可减少黄斑裂孔手术后的视野缺损。”眼外科激光。

Kuroki AM, Kitaoka T, Taniguchi H, Amemiya T.: "Hyperbaric oxygen therapy reduces visual field defect after macular hole surgery."Opthalmic Surg Lasers. 33. 200-206 (2002)

Kuroki AM、Kitaoka T、Taniguchi H、Amemiya T.：“高压氧疗法可减少黄斑裂孔手术后的视野缺损。”眼外科激光。

芦忠陽, 岸川泰宏, 津田恭央, 北岡隆, 雨宮次生: "黄斑部内境界膜の微細構造とその元素分析"臨眼. 57. 281-284 (2003)

Tadayo Ashi、Yasuhiro Kishikawa、Yasuo Tsuda、Takashi Kitaoka、Tsugio Amemiya：“黄斑区限制膜的精细结构及其元素分析”，影像杂志 57. 281-284 (2003)。