Exploration of genes and the functions affected by serum calcium-decreasing factor (caldecrin)

钙降蛋白是从胰腺中纯化的血清降钙因子，是一种凝乳胰蛋白酶型丝氨酸蛋白酶。钙钙素以形式合成，由细胞分泌。在探索钙decrin功能的研究项目中，我们发现了以下结果：1)钙脱蛋白抑制骨吸收，但不影响其蛋白酶活性。与大鼠钙decrin对应的氨基酸序列（aa71-79）负责抑制钙decrin的骨吸收。2)钙decrin在骨骼肌中表达，其mRNA表达量随着肌肉分化的进展而增加。3)成年大鼠脑钙decrin的检测。钙降蛋白基因在出生后1-28天在大脑中组成性表达。全脑包括嗅球、大脑、海马、丘脑和小脑均检测到钙脱蛋白mRNA。这些结果表明钙降蛋白是一种多功能蛋白酶。

Caldecrin, a serum calcium-decreasing factor that we purified from pancreas, is a chymotrypsin-type serine protease. Caldecrin is synthesized as proform and secreted from the cells. In the course of research project that explore the function of caldecrin, we found the results as follows; 1) caldecrin suppressed bone resorption without its protease activity. The amino acid sequence corresponding to rat caldecrin (aa71-79) is responsible for the suppression of bone resorption by caldecrin. 2) Caldecrin was expressed in the skeletal muscle and its mRNA expression was increased with the progress of muscle differentiation. 3) Caldecrin detected in adult rat brain.The caldecrin gene was constitutively expressed during postnatal days 1-28 in the brain. The caldecrin mRNA was detected in the whole brain, including the olfactory bulb, cerebrum, hippocampus, thalamus and cerebellum.These results indicate that caldecrin is multi-functional protease.