A study of molecular biology to endcrine status on the development and proliferation of oral carcinomas

口腔癌发生、增殖的内分泌状态的分子生物学研究

• 批准号: 11672021
• 负责人: OZONO Satoru
• 金额: $ 1.66万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672021/
• 关键词: estrogen receptor; progesterone receptor; oral squamous cell carcinoma; RT-PCR; ERmRNA; 口腔扁平上皮癌; エストロゲン受容体; プロゲステロン受容体; 免疫組織化学; (1)口腔扁平上皮癌; (2)エストロゲン受容体; (3)プロゲステロン受容体; (4)エストロゲン調節蛋白; (5)免疫組織化学

Estrogen receptor (ER) and progesterone receptor (PgR) expression in paraffin-embedded tissues from 103 patients with oral squamous cell carcinomas (SCC) was studied immunohistochemically using the anti-ER and anti-PgR antibodies. The 103 patients (60 males and 43 females) were 31-to 89-year-old (median age 62.8) at first diagnosis. Primary sites consisted of the tongue in 34 patients, gingiva in 27 patients, buccal mucosa in 6 patients, floor of mouth in 8 patients, maxillary sinus in 11 patients and palate in 7 patients.Of 103 tumors, 54 (58.2%) were ER positive. In these specimens, in agreement with the results seen with the breast cancer tissue used as positive control, strongly-stained cells, in which the ER-immunoreactivity was located in the cell nucleus, were detected. Accordingly, the cases were classed as ER-positive or ER-negative by scoring them on the basis of the visually estimated percentage of tumor cells showing positive nuclear staining, followed by an evaluation usin … More g a 10% cut-off level. In seventeen tumors approximately 10-50% of tumor cells expressed and in thirty-seven tumors over 50% of tumor cells were positive. There were 42 tumors with no nuclear staining and 7 tumors in which a few (<10%) tumor cells were stained. Of 54 ER-positive oral SCCs, only 10 (9.7%) were positive for nuclear PgR : one expressed PgR in more than 75% of tumor cell nuclei, the other in 10-50% of tumor cell nuclei. Histologically, PgR-positive tumors consisted of 9 well-differentiated carcinomas and 1 moderately differentiated type of oral SCC.ER immunoreactivity correlated with the presence of ERmRNA in tumor tissue analyzed using the reverse transcription polymerase chan reaction.These results suggest that a mechanism similar to the ovarian steroid-mediated induction and proliferation of the breast cancer also operates in oral SCC, speculating the possible clinical use of endocrine therapy for the management of this type of tumor. It is very important to determine whether differences in prognosis exist between patients with ER-positive and ER-negative tumors. Less

采用免疫组织化学方法对103例口腔鳞状细胞癌石蜡包埋组织中雌激素受体（ER）和孕激素受体（PgR）的表达进行了研究。103例患者（男60例，女43例）初诊时年龄31 ~ 89岁，中位年龄62.8岁。原发部位包括舌34例、龈27例、颊黏膜6例、口腔底8例、上颌窦11例、上颚7例。103例肿瘤中，54例（58.2%）为ER阳性。在这些标本中，与作为阳性对照的乳腺癌组织的结果一致，检测到强烈染色的细胞，其中er免疫反应性位于细胞核中。因此，根据肉眼估计的核染色阳性的肿瘤细胞百分比，将病例分为er阳性或er阴性，然后使用…More g 10%的截止水平进行评估。在17个肿瘤中，大约10-50%的肿瘤细胞表达，在37个肿瘤中，超过50%的肿瘤细胞呈阳性。无核染色42例，少量（<10%）肿瘤细胞染色7例。在54例er阳性的口腔SCCs中，只有10例（9.7%）核PgR阳性：1例在超过75%的肿瘤细胞核中表达PgR，另1例在10-50%的肿瘤细胞核中表达PgR。组织学上，pgr阳性肿瘤包括9例高分化癌和1例中分化型口腔SCC。利用逆转录聚合酶chan反应分析肿瘤组织中ERmRNA的存在与ER免疫反应性的相关性。这些结果表明，类似于卵巢类固醇介导的乳腺癌诱导和增殖的机制也在口服SCC中起作用，推测内分泌治疗在临床上可能用于治疗这类肿瘤。确定er阳性和er阴性肿瘤患者预后是否存在差异是非常重要的。少

项目成果

小園知 他: "咀嚼機能と老化:特に高齢期の高次機能に及ぼす影響"歯基礎誌. 42・5. 139 (2000)

Satoshi Kozono 等人：“咀嚼功能和衰老：特别是其对老年高级功能的影响”《牙科科学杂志》42・5（2000 年）。

Yamamoto,T.: "Origin of primary sensory neurons innervating the buccal stretch receptor"J.Dent.Res.. 78・1. 49-53 (1999)

Yamamoto, T.：“支配颊牵张受体的初级感觉神经元的起源”J.Dent.Res.. 78・1（1999）。

Onozuka, M., Watanabe, K., Mirbod, SM., Ozono, S.Ozono S.et al.: "Reduced mastication stimulates impairment of spatial memory and degeneration of hippocampal neurons in aged SAMP8 mice."Brain Res.. 826. 148-153 (1999)

Onozuka, M.、Watanabe, K.、Mirbod, SM.、Ozono、S.Ozono S.等人：“咀嚼减少会刺激老年 SAMP8 小鼠的空间记忆受损和海马神经元退化。”Brain Res.. 826

本間義郎: "口腔粘膜多発癌の臨床病理学的特徴と背景因子"口腔腫瘍. 11・3. 151-161 (1999)

Yoshiro Honma：“多发性口腔粘膜癌的临床病理学特征和背景因素”口腔肿瘤11・3（1999）。

Onozuka,M.: "Reduced mastication stimulates impairment of spatial memory and degeneration oh hippocampal neurons in aged SAMP8 mice"Brain Res.. 826. 148-153 (1999)

Onozuka,M.：“咀嚼减少会刺激老年 SAMP8 小鼠的空间记忆损伤和海马神经元退化”Brain Res.. 826. 148-153 (1999)